Abstract
Cholesterol is a waxy steroid metabolite substance and plays an important role in the brain, nervous, and immune systems of humans. The level of cholesterol in human serum has become an important index for clinical diagnosis and prevention of cardiovascular disease. In this study, a highly sensitive electrochemical biosensor for cholesterol measurement has been developed based on reduced graphene oxide-chitosan-ferrocene carboxylic acid/platinum nanoparticle (RGO-CS-Fc/Pt NPs) modified screen-printed carbon electrode (SPCE). Under the effect of cholesterol oxidase (CHOD) and cholesterol esterase (CHER), the cholesterol was oxidized to generate hydrogen peroxide (H2O2), which can be disintegrated into H2O with the synergistic catalysis of RGO-CS-Fc/Pt NPs and produce the redox response current of H2O2 that could be determined by electrochemical amperometric (i-t) method. The prepared cholesterol biosensor was achieved by integrating of the high electrocatalytic efficiency of Pt NPs, high electronic conductivity and large surface area of RGO, the reversible electrochemical behavior of Fc, and good biocompatibility of CS. The measured of redox response current and cholesterol concentration have a good linearity in the range of 0.5–4.0 mg/mL, low detection limit of 5.70 μg/mL (S/N = 3), and good sensitivity of 0.871 nA/mM/cm2. Moreover, the RGO-CS-Fc/Pt NPs cholesterol biosensor exhibited excellent specificity, acceptable reproducibility, and higher recoveries in the detection of clinical serum samples, showing great potential for clinical diagnosis.
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Funding
This work was supported by the National Nature Science Foundation of China (nos. 81460451 and 8176053), the National Science Foundation of Guangxi Province of China (2016GXNSFAA380080), and the Innovation Project of GUET Graduate Education (nos. 2017YJCX90 and 2017YJCX100).
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This article is part of the topical collection: “Nanoparticles in Biotechnology and Medicine"
Xiaoshan (Sean) Zhu, University of Nevada, Guest Editor
Research highlights
• A cholesterol electrochemical biosensor based on RGO-CS-Fc/Pt NPs was developed.
• The RGO-CS-Fc/Pt NPs cholesterol biosensor was characterized by SEM, XPS, and CV.
• The RGO-CS-Fc/Pt NPs cholesterol biosensor demonstrated a good linear calibration curve with R2 of 0.99897.
• The RGO-CS-Fc/Pt NPs cholesterol biosensor showed lower detection limit, higher specificity, acceptable stability, and good recovery.
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Li, G., Zeng, J., Zhao, L. et al. Amperometric cholesterol biosensor based on reduction graphene oxide-chitosan-ferrocene/platinum nanoparticles modified screen-printed electrode. J Nanopart Res 21, 162 (2019). https://doi.org/10.1007/s11051-019-4602-6
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DOI: https://doi.org/10.1007/s11051-019-4602-6