Summary
The physical properties of the transducing DNA of HT-phage mutants are described. It could be shown that the increased frequency of transducing particles is not due to replication of such structures formed at a normal rate, but rather to an increased rate of their formation. Further it turned out that density and the sedimentation coefficient of transducing DNA of HT-mutants is comparable to transducing DNA of wild type phages. It could be confirmed that there is also a contribution of about 10% newly synthesized DNA to the bacterial fragment. Therefore it is concluded that the mechanism which leads to transducing particles in HT-mutants is the same as in wild type phages.
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Communicated by P. Starlinger
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Schmieger, H., Backhaus, H. The origin of DNA in transducing particles in P22-mutants with increased transduction-frequencies (HT-mutants). Molec. Gen. Genet. 120, 181–190 (1973). https://doi.org/10.1007/BF00267246
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DOI: https://doi.org/10.1007/BF00267246