Abstract
Purified rat ceruloplasmin is extraordinarily unstable in storage at −70 °C. In a 20 mM phosphate buffer, pH 7.0, the ferroxidase and amine oxidase of ceruloplasmin are over 90% inactivated within two weeks. Holoceruloplasmin stored for three months in a 20 mM barbital buffer (or acetate buffer), pH 7.0 (or pH 5.5) was transformed into an apo-protein and amine (o-dianisidine) oxidase of ceruloplasmin was inactivated by 50–55%. The patterns of ferroxidase activity loss were similar to those of amine oxidase activity loss. On the contrary, when holoceruloplasmin was mixed with rat serum albumin, transformation into apoceruloplasmin was significantly prevented in a 20 mM barbital buffer, pH 7.0 (or 20 mM acetate buffer, pH 5.5). Consequently, ferroxidase and amine oxidase activities of ceruloplasmin were not inactivated and the immunochemical reactivity was not changed. These results can be applied for laboratorial and clinical purposes.
Similar content being viewed by others
References
Calabrese L, Carbonaro M, Musci G (1988) J. Biol. Chem. 263: 6480-6483.
Cousins RJ (1985) Physiol. Rev. 65: 238-309.
Ehrenwald E, Chisolm GM, Fox PL (1994) J. Clin. Invest. 93, 1493-1501.
Fleming RE, Giltin JD (1990) J. Biol. Chem. 265: 7701-7707.
Fleming RE, Whitman IP, Giltin JD (1991) Am. J. Physiol. 260: L68-L74.
Giltin JD, Schroeder JJ, Lee-Ambrose LM, Cousinns RJ (1992) Biochem. J. 282: 835-839.
Halliwell B., Gutteridge JMC (1990) Arch. Biochem. Biophys. 280: 1-8.
Johnson DA, Osaki S, Frieden E (1967) Clin. Chem. 13: 142-150.
Kim IG, Park SY (1998) FEBS Lett. 437: 293-296.
Kim IG, Park SY, Kim KC (1998) Biochem. Mol. Biol. Int. 45: 599-608.
Kim IG, Park SY, Kim KC, Yum JJ (1998) FEBS Lett. 431: 473-475.
Kim IG, Shim HW, Lee KS (1998) Biotechnol. Techn. 12: 133-136.
Leatherbarrow RJ, Dean DG (1980) Biochem. J. 189: 27-34.
Musci G, Patti MCB, Fagiolo U, Calabrase L (1993) J. Biol. Chem. 268: 13388-1339.
Osaki S, Jhonson A, Friden E (1966) J. Biol. Chem. 241: 2746-2757.
Roeser HP, Lee GR, Nacht S, Cartwrite GE (1970) J. Clin. Invest. 49: 2408-2471.
Ryan PT, Grover TA, Aust SD (1992) Arch. Biochem. Biophys. 293: 1-8.
Ryden L (1984) Ceruloplasmin. In: Lontin R, ed. Copper Proteins and Copper Enzymes, Vol. III. Boca Raton, FL: CRC Press, pp. 37-100.
Ryden L, Bjork I (1976) Biochemistry 15: 3411-3417.
Schosinsky KF, Lehmann HP, Beeler MF (1974) Clin. Chem. 20: 1556-1563.
Semsei I (1991) Gerontology 37: 199-207.
Winyard PG, Hider RC, Brailsford S, Drake AF, Lunec J, Blake DR (1989) Biochem. J. 258: 435-445.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kim, I.G., Park, S.Y. Rat albumin inhibits the formation of apoceruloplasmin during storage. Biotechnology Techniques 13, 469–473 (1999). https://doi.org/10.1023/A:1008956818851
Issue Date:
DOI: https://doi.org/10.1023/A:1008956818851