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The packaging initiation site of phage P22

Analysis of packaging events by transduction

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Summary

P22 lysates were grown on Salmonella strains carrying P22 prophages deleted to various extents. Transducing bacterial markers at both sides of the prophage insertion site it could be shown that: (i) transduction of markers can be enhanced by the prophage pac site; (ii) the recognition signal pac is in the area of gene 3 on the phage genome and thus close to the cutting site(s); (iii) transposon Tn10 may also act as a signal for packaging initiation; (iv) (at least) Tn10 initiates packaging sequences in both directions.

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Authors and Affiliations

  1. Institut für Genetik und Mikrobiologie der Universität München, Maria-Ward-Strasse 1 a, D-8000, München 19, Federal Republic of Germany

    Barbara Kufer, Horst Backhaus & Horst Schmieger

Authors
  1. Barbara Kufer
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  2. Horst Backhaus
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  3. Horst Schmieger
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Communicated by H. Saedler

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Kufer, B., Backhaus, H. & Schmieger, H. The packaging initiation site of phage P22. Molec Gen Genet 187, 510–515 (1982). https://doi.org/10.1007/BF00332636

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  • DOI: https://doi.org/10.1007/BF00332636

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