Summary
Utilizing the DNA sequence of the metalloprotease fromSerratia strain E-15, we isolated and sequenced the homologous gene fromSerratia strain SM6. These two genes are similar at both the DNA and protein sequence level. Expression of the protease gene inEscherichia coli was achieved by use of thelac promoter. This resulted in the production and excretion of an immunologically detectable but inactive protein of slightly higher molecular weight than that fromSerratia. We introduced the cloned gene into previously described protease mutants. The observed pattern of protease expression suggested that these mutations fall into three classes.
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Communicated by J. Lengeler
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Braunagel, S.C., Benedik, M.J. The metalloprotease gene ofSerratia marcescens strain SM6. Mol Gen Genet 222, 446–451 (1990). https://doi.org/10.1007/BF00633854
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DOI: https://doi.org/10.1007/BF00633854