Recognition of the small regulatory RNA RydC by the bacterial Hfq protein

Please always quote using this URN: urn:nbn:de:bvb:20-opus-114191
  • Bacterial small RNAs (sRNAs) are key elements of regulatory networks that modulate gene expression. The sRNA RydC of Salmonella sp. and Escherichia coli is an example of this class of riboregulators. Like many other sRNAs, RydC bears a 'seed' region that recognises specific transcripts through base-pairing, and its activities are facilitated by the RNA chaperone Hfq. The crystal structure of RydC in complex with E. coli Hfq at 3.48 angstrom resolution illuminates how the protein interacts with and presents the sRNA for target recognition.Bacterial small RNAs (sRNAs) are key elements of regulatory networks that modulate gene expression. The sRNA RydC of Salmonella sp. and Escherichia coli is an example of this class of riboregulators. Like many other sRNAs, RydC bears a 'seed' region that recognises specific transcripts through base-pairing, and its activities are facilitated by the RNA chaperone Hfq. The crystal structure of RydC in complex with E. coli Hfq at 3.48 angstrom resolution illuminates how the protein interacts with and presents the sRNA for target recognition. Consolidating the protein-RNA complex is a host of distributed interactions mediated by the natively unstructured termini of Hfq. Based on the structure and other data, we propose a model for a dynamic effector complex comprising Hfq, small RNA, and the cognate mRNA target.show moreshow less

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Metadaten
Author: Daniela Dimastrogiovanni, Kathrin S. Fröhlich, Katarzyna J. Bandyra, Heather A. Bruce, Susann Hohensee, Jörg Vogel, Ben F. Luisi
URN:urn:nbn:de:bvb:20-opus-114191
Document Type:Journal article
Faculties:Medizinische Fakultät / Institut für Molekulare Infektionsbiologie
Language:English
Parent Title (English):eLife
ISSN:2050-084X
Year of Completion:2014
Volume:3
Issue:e05375
Source:eLife 2014; 3 : e05375. DOI: 10.7554/eLife.05375
DOI:https://doi.org/10.7554/eLife.05375
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Tag:C-Terminus; Escherichia coli-Hfq; Hfq; SM-like protein; chaperone Hfq; crystal-structures; gene regulation; interaction surfaces; messenger-RNA; natively unstructured protein; noncoding RNAs; protein-RNA recognition; small RNA; soluble-RNAs; target recognition
Release Date:2015/06/26
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung