Umfang:
Online-Ressource (IX, 654 p. 120 illus., 42 illus. in color, digital)
ISBN:
9783642274268
Serie:
RNA Technologies
Inhalt:
Despite a half century of structural, biophysical and biochemical investigations of ribonucleic acids, they are still mysterious. RNAs stand at fertile crossroads of disciplines, integrating concepts from genomics, proteomics, dynamics as well as biochemistry and molecular biology. From 20 years it is clear, that genetic regulation of eukaryotic organisms has been misunderstood for the last years that the expression of genetic information is effected only by proteins. Basic understanding of nucleic acids has enhanced our foundation to probe novel biological functions. This is especially evident for RNA molecules whose functionality, maturation, and regulation require formation of correct secondary structure through encoded base-pairing interactions.
Anmerkung:
Description based upon print version of record
,
From Nucleic Acids Sequences to Molecular Medicine; Preface; Nucleic Acids Meet Human Genetic Medicine; Contents; RNAissance; 1 From So Simple a Beginning; 2 Transitions in Slow Motion; 3 RNA First; 4 From RNA to RNP World; 5 Late DNA; 6 Rays of Light in the Darkness; 7 The Avalanche; 8 Is All that Is Transcribed an RNA?; 9 Life Without RNA?; 10 RNA in Human Disease and Therapy; References; Nucleic Acids as Therapeutics; 1 Introduction; 2 DNA-Based Therapeutics; 2.1 Plasmids; 2.2 Oligonucleotides for Antisense and Antigene Applications; 2.3 Aptamers; 2.4 DNAzymes; 3 RNA-Based Therapeutics
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3.1 RNA Aptamers3.2 RNA Decoys; 3.3 Antisense RNA; 3.4 Ribozymes; 3.5 Small Interfering RNAs; 3.6 MicroRNA; 4 Gene Transfer Technologies; 4.1 Mechanical and Electrical Techniques; 4.2 Vector-Assisted Delivery Systems; 4.2.1 Viral Delivery Systems; 4.2.2 Nonviral Delivery Systems; 5 Current Status of Gene Therapy Research; 6 Recent Developments in Gene Therapy; References; Thio Effects as a Tool for Mechanistic Studies of the Cleavage of RNA Phosphodiester Bonds: The Chemical Basis; 1 Introduction
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2 The Effect of Nonbridging Thio Substitution on Metal-Ion-Independent Cleavage and Isomerization of RNA Phosphodiester Linkag...3 The Effect of Nonbridging Thio Substitution on Metal-Ion-Dependent Cleavage of RNA Phosphodiester Linkages; 4 The Effect of Thio Substitution on the Hydrolysis Ribonucleoside 2,3-Cyclic Phosphates; 5 The Effect of Bridging Thio Substitution on the Cleavage and Isomerization of RNA Phosphodiester Linkage; 6 Thio Effects on Reactions of Mono- and Triester Congeners of Ribonucleoside 3-Phosphodiesters; References
,
Off-Target Effects and Safety Aspects of Phosphorothioate Oligonucleotides1 Introduction; 1.1 Oligonucleotide Therapeutics; 1.2 Clinical Development; 2 Phosphorothioate Antisense Oligonucleotides; 2.1 Chemical Background; 2.2 Clinical Trials; 2.3 Pharmacokinetics and Toxicology; 3 Specificity of Phosphorothioates; 3.1 Off-Target Effects; 3.2 Molecular Mechanisms of Off-Target Effects in Vitro; 3.3 In Vivo Correlation; 4 Off-Target Effects of Other Modifications; 5 Summary; References; Oligonucleotide Conjugates: Rationale, Synthesis, and Applications; 1 Introduction
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2 Oligonucleotide Conjugates3 Syntheses of Oligonucleotide Conjugates; 3.1 Solid-Phase Conjugate Synthesis; 3.1.1 Incorporation of Promoiety During the Solid-Phase Synthesis; 3.1.2 In-Line or Stepwise Solid-Phase Conjugate Synthesis; 3.1.3 On-Support Fragment Conjugation; 3.2 Solution-Phase Conjugate Synthesis; 3.2.1 Amide and Thiourea Linkages; 3.2.2 Imine, Hydrazone, and Oxime Linkages; 3.2.3 Disulfide, Thioether, Thiazolidine, Native Ligation, and Metal-Thiol Linkages; 3.2.4 Diels-Alder [4+2] Cycloadditions; 3.2.5 Staudinger Ligation and 1,3-Dipolar Cycloadditions
,
4 Oligonucleotide Conjugates in Early Development
,
RNAissance
,
Nucleic Acids as Therapeutics
,
Thio Effects as a Tool for Mechanistic Studies of the Cleavage of RNA Phosphodiester Bonds: The Chemical Basis
,
Off-Target Effects and Safety Aspects of Phosphorothioate Oligonucleotides
,
Oligonucleotide Conjugates: Rationale, Synthesis, and Applications
,
CRISPR: A Bacterial Immunity System Based on Small RNAs
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Antisense Technology: From Unique Laboratory Tool to Novel Anticancer Treatments
,
Antisense-Mediated Reduction of Eukaryotic Noncoding RNAs
,
Antisense Oligonucleotides in the Treatment of Malignant Gliomas
,
Natural Antisense Transcripts Mediate Regulation of Gene Expression
,
Activation and Deactivation of Antisense and RNA Interference Function with Light
,
Ribozymes as Molecular Biology Reagents
,
RNA Technologies for Mitochondrial Genetics
,
RNA-Based Therapies for Inherited Metabolic Diseases
,
RNA-Cleaving DNA Enzymes and Their Potential Therapeutic Applications as Antibacterial and Antiviral Agents
,
Hammerhead Ribozymes Against Virus and Viroid RNAs
,
Suppression of Hepatitis C Viral Genome Replication with RNA-Cleaving Deoxyribozyme
,
Design of Synthetic shRNAs for Targeting Hepatitis C: A New Approach to Antiviral Therapeutics
,
The Diverse Active Sites in Splicing, Debranching, and MicroRNA Processing Around RNA Phosphodiester Bonds
,
MicroRNAs and Their Antagonists as Novel Therapeutics
,
microRNAs in Human Diseases and Viral Infections
,
Dysregulation of MicroRNA Expression and Human Diseases?
,
Noncoding RNAs: Identification of Cancer-Associated MicroRNAs
,
Targeting Non-coding RNAs for Cancer Therapy
,
MicroRNA Pathways in Drosophila
,
Viroids: The Smallest Known Infectious Agents Cause Accumulation of Viroid-Specific Small RNAs
Weitere Ausg.:
ISBN 9783642274251
Weitere Ausg.:
Buchausg. u.d.T. From nucleic acids sequences to molecular medicine Berlin : Springer, 2012 ISBN 9783642274251
Weitere Ausg.:
ISBN 3642274250
Sprache:
Englisch
Fachgebiete:
Biologie
Schlagwort(e):
RNS
;
Molekulare Medizin
;
Aufsatzsammlung
DOI:
10.1007/978-3-642-27426-8
URL:
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