UID:
almahu_9948025844702882
Format:
1 online resource (994 p.)
Edition:
1st ed.
ISBN:
1-281-04832-1
,
9786611048327
,
0-08-053686-7
,
1-4356-0812-7
Series Statement:
Techniques in the behavioral and neural sciences ; v. 13
Content:
The book gives a broad overview of recombinant DNA techniques for the behavioral neuroscientist, with illustrative examples of applications. Species covered include rodents (mainly mice), Drosophila melanogaster, Caenorhabditis elegans and Danio rerio. Experimental techniques required to characterize the behavioral phenotypes of mutant animals is provided. Several aspects of novel molecular-genetic techniques are overviewed and possible research strategies are explained. The sections of the book start with general descriptions of techniques followed by illustrative examples.
Note:
Description based upon print version of record.
,
Front Cover; Techniques in the Behavioral and Neural Sciences; Copyright Page; Contents; Dedication; Preface. Behavioral neurogenetics: the new synthesis; Acknowledgements; List of Contributors; Section 1: Neurobehavioral Informatics and Statistics; Chapter 1.1. The multi-dimensional database requirements of brain information in the era of rapid gene identification; Chapter 1.2. Bioinformatics and neuroscience in the post-genomic era; Chapter 1.3. PsycheMap- an online database of psychiatric genetics linkage, association, and genome mapping projects
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Chapter 1.4. Experimental design and statistical inferenceSection 2: Searching for New Genes; Section 2.1: Spontaneous and Induced Mutations with Effects on Neural and Behavioral Traits; Chapter 2.1.1. Mapping single locus mutations in mice: towards gene identification of neurological traits; Chapter 2.1.2. Experimental strategies for mapping quantitative trait loci (QTL) analysis in laboratory animals; Chapter 2.1.3. Linkage strategies for mapping genes for complex traits in man; Chapter 2.1.4. Genetic association studies in behavioral neuroscience
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Chapter 2.1.5. DNA pooling in allelic association studiesChapter 2.1.6. Research strategies for the analysis of neurological mutants of the mouse; Chapter 2.1.7. Genetic dissection of mouse behavior using induced mutagenesis; Chapter 2.1.8. Mutagenesis in zebra fish: studying the brain dopamine systems; Chapter 2.1.9. Behavioral and electrophysiological screens for isolating zebra fish mutants with visual system defects; Section 2.2: Finding Genes with Phenotypical Effects on Neural and Behavioral; Chapter 2.2.1. Subtractive cDNA hybridization and the brain: then, now, and tomorrow
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Chapter 2.2.2. Applying differential display to brain researchChapter 2.2.3. Brain region-specific genes: the hippocampus; Chapter 2.2.4. Application of real-time RT-PCR for quantification of gene expression; Chapter 2.2.5. Analyzing genomic DNA discordance between monozygotic twins; Section 3: Manipulating Known Genes; Section 3.1: Transgenic Approaches to Rodents; Chapter 3.1.1. Embryonic stem cells and gene targeting; Chapter 3.1.2. Generation of transgenic mice by pronuclear DNA injection
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Chapter 3.1.3. Brain region-specific and temporally restricted gene knockout using the Cre recombinase systemChapter 3.1.4. Regulated temporal and spatial expression of mutants of CaMKII and calcineurin with the tetracycline-controlled transactivator (tTA) and reverse tTA (rtTA) systems; Chapter 3.1.5. The use of targeted point mutants in the study of learning and memory; Chapter 3.1.6. Genetic dissection of a postsynaptic multiprotein complex controlling synaptic plasticity and learning in the mouse
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Chapter 3.1.7. Molecular genetic analysis of glutamate receptor function in long-term potentiation in the mouse hippocampus
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English
Additional Edition:
ISBN 0-444-50239-4
Language:
English
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