UID:
almafu_9958077210002883
Umfang:
1 online resource (507 p.)
ISBN:
1-281-05887-4
,
9786611058876
,
0-08-053639-5
Serie:
Methods in molecular genetics, v. 6
Inhalt:
Microbial Gene Techniques is a practical laboratory guide to current techniques of molecular biology and genetics. The focus of the volume is on microbial cells, particularly eukaryotic microbes and bacteria, as well as plasmids and bacteriophages.* * Methods presented for ease of use and ready adaptation to new systems.* Detailed protocols included for:* Eukaryotic microbes - protozoan parasites (forward and reverse genetics, genome analysis), filamentous fungi (chromosome and gene analysis)* Yeast chromosomes - YACs, genome mapping, transcription factors,
Anmerkung:
Description based upon print version of record.
,
Front Cover; Microbial Gene Techniques; Copyright Page; Table of Contents; Contributors to Volume 6; Preface; Volumes in Series; Section I: Molecular Genetics of Eukaryotic Microbes; Chapter 1. Forward and Reverse Genetics in the Study of the Obligate, Intracellular Parasite Toxoplasma gondii; Chapter 2. Techniques Associated with Protozoan Parasite Genome Analysis; Chapter 3. Aspergillus nidulans as a Model System for Cell and Molecular Biology Studies; Chapter 4. Special Molecular Techniques for Study of Filamentous Fungi
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Section II: Yeast Chromosomes: Transcription, Recombination, ReplicationChapter 5. In Vitro Fragmentation of Yeast Chromosomes and Yeast Artificial Chromosomes at Artificially Inserted Sites and Applications to Genome Mapping; Chapter 6. Basic Analysis of Transcription Factor Binding to Nucleosomes; Chapter 7. Experimental Analysis of Transcription Factor-Nucleosome Interactions; Chapter 8. In Vivo Analysis of Nucleosome Structure and Transcription Factor Binding in Saccharomyces cerevisiae; Chapter 9. Mapping of Yeast Nucleosomes in Vivo
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Chapter 10. Novel Assays for Ligation and Cleavage Activities of Site-Specific RecombinasesChapter 11. Physical Monitoring of Mitotic and Meiotic Recombination in Saccharomyces cerevisiae; Chapter 12. Genetic Dissection of Complex Heteromultimeric Enzymes: The Case of Yeast DNA-Dependent RNA Polymerases; Chapter 13. Pheromone Procedures in Fission Yeast; Section III: Bacterial Gene Structure and Regulation; Chapter 14. Use of DNA Methylation Deficient Strains in Molecular Genetics; Chapter 15. Identifying the Ends of Infrequent RNA Molecules in Bacteria
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Chapter 16. Identification of rcs Genes in Escherichia coli 09:K30:H12 and Involvement in Regulation of Expression of Group IAK30 Capsular PolysaccharideChapter 17. Genetic Mapping in Bacillus subtilis; Chapter 18. Molecular and Biochemical Methods for Studying Chemotaxis in Bacillus subtilis; Chapter 19. Cloning of Thermomonospora fusca Cellulase Genes in Escherichia coli and Streptomyces lividans; Chapter 20. Computational Analyses Aiding Identification and Characterization of Proteins, Genes, and Operons; Section IV: Plasmids and Phages: Replication,Transcription, Assembly
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Chapter 21. Techniques to Isolate Specialized Templates for in Vitro Transcription AssaysChapter 22. Protein-Protein Interactions of DNA-Binding Proteins: Studies on the Replication Initiator Protein, Rep A, of Plasmid P1; Chapter 23. Transcriptional Regulators: Protein-DNA Complexes and Regulatory Mechanisms; Chapter 24. Use of Coliphage a and Other Bacteriophages for Molecular Genetic Analysis of Erwinia and Related Gram-Negative Bacteria; Chapter 25. Isolation, Purification, and Function of Assembly Intermediates and Subviral Particles of Bacteriophages PRD1 and ø6; Index
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English
Weitere Ausg.:
ISBN 0-12-044308-2
Sprache:
Englisch
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