UID:
almafu_9960073241802883
Umfang:
1 online resource (435 p.)
ISBN:
1-281-05921-8
,
9786611059217
,
0-08-053640-9
Serie:
Methods in molecular genetics ; v. 7
Inhalt:
Viral Gene Techniques is a practical laboratory guide to current techniques of molecular biology and genetics. The volume is concerned with methods for the analysis of viral genes and chromosomes: DNA viruses and RNA viruses including HIV are discussed.* Methods presented for ease of use and ready adaptation to new systems* Detailed experimental protocols included for:* Viral vectors - construction and use of DNA virus vectors (adenovirus, adeno-associated virus, vaccinia virus, Epstein - Barr virus)* DNA viruses - virus/host interactions, viral chromosomes , transcript
Anmerkung:
Description based upon print version of record.
,
Front Cover; Viral Gene Techniques; Copyright Page; Table of Contents; Contributors to Volume 7; Preface; Volumes in Series; Section I: Viral Vectors; Chapter 1. Adeno-Associated Virus: Integrating Vectors for Human Gene Therapy; Chapter 2. Techniques for Human Adenovirus Vector Construction and Characterization; Chapter 3. Construction and Isolation of Recombinant Adenoviruses with Gene Replacements; Chapter 4. Construction and Use of Recombinant Vaccinia Virus Vectors; Chapter 5. Epstein-Barr Viral Plasmid Vectors and Their Amplifiable Derivatives; Section II: DNA Viruses
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Chapter 6. Methods to Study Epstein-Barr Virus and p53 Status in Human CellsChapter 7. The SV40 Minichromosome; Chapter 8. Site-Specific Integration of Multigenic Shuttle Plasmids into the Herpes Simplex Virus Type 1 Genome Using a Cell-Free Cre-lox Recombination System; Chapter 9. Strategies for Studying Transcription Regulation of Neurotropic Viruses in the Central Nervous System: Lesson from JC Virus; Chapter 10. Analysis of the Nuclear DNA-Binding Activity in Cells Overexpressing Hepatitis B Viral X and 3'-Truncated S Transactivators
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Chapter 11. Investigations on Virus-Host Interactions: An Abortive System Section III: Human Immunodeficiency Virus/Retroviruses; Chapter 12. Quantitation of Virus Stocks Produced from Cloned Human Immunodeficiency Virus DNA; Chapter 13. Quantitation of HIV-1 RNA in Plasma or Serum Samples; Chapter 14. Detection of Cell Fusion Mediated by the Envelopes of Human Retroviruses by Transactivation of a Reporter Gene; Chapter 15. Assembly of Recombinant Retroviral Gag Precursors into Pseudovirions in the Baculovirus-Insect Cell Expression System
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Chapter 16. Retrovirus Reverse Transcription and Integration Chapter 17. In Vitro Binding and Transcription Assays Using the Human T-Cell Leukemia Virus Type I Tax Protein; Chapter 18. Polymerase Chain Reaction-Based Techniques for Utilizing Retroviruses as Cell Lineage Markers; Section IV: RNA Viruses; Chapter 19. New Methods to Study Poliovirus Assembly and Encapsidation of Genomic RNA; Chapter 20. Molecular Studies of Hepatitis Delta Virus RNAs; Chapter 21. Systems to Express Recombinant RNA Molecules by the Influenza A Virus Polymerase in Vivo
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Chapter 22. Analysis of Membrane Association in Vivo and Targeting of Viral Proteins in Polarized Epithelial Cells Chapter 23. Cloning of Viral Double-Stranded RNA Genomes by Single Primer Amplification; Chapter 24. Using the RNA-Capture Assay to Assess the RNA-Binding Activity of Viral Proteins; Chapter 25. Protein RNA-Binding Activity Measured by Northwestern Blot Analysis: The Interferon-Inducible RNA-Dependent Protein Kinase PKR; Erratum; Index
,
English
Weitere Ausg.:
ISBN 0-12-044309-0
Sprache:
Englisch
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