UID:
almahu_9948026577002882
Umfang:
1 online resource (739 p.)
Ausgabe:
1st ed.
ISBN:
1-281-05889-0
,
9786611058890
,
0-08-050779-4
Serie:
Separation science and technology ; v. 2
Inhalt:
It is generally recognized that the commercial success of biotechnology products is highly dependent on the successful development and application of high-powered separation and purification methods. In this practical and authoritative handbook, the separation of proteins, nucleic acids, and oligonucleotides from biological matrices is covered from analytical to process scales. Also included in a chapter on the separation of monoclonal antibodies, which have found numerous uses as therapeutic and diagnostic agents. Analytical techniques include an interesting montage of chromatographic methods
Anmerkung:
Description based upon print version of record.
,
Cover; CONTENTS; PREFACE; Chapter 1. Bioseparations: An Overview; I. Introduction; II. Analytical Methodologies; III. Separation and Purification Methods; IV. Other Important Considerations; Reference; Chapter 2. Analysis of Protein Impurities in Pharmaceuticals Derived from Recombinant DNA; I. Introduction; II. Protein Impurity Analysis; III. Experimental Summary; IV. Case Studies; References; Chapter 3. Physicochemical Factors in Polypeptide and Protein Purification and Analysis by High-Performance Liquid Chromatographic Techniques: Current Status and Challenges for the Future
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I. IntroductionII. Basic Chromatographic Terms and Concepts; III. The Chemical Structure of Polypeptides and Proteins; IV. Physicochemical Factors That Underpin Ligate Interactions with Polypeptides and Proteins in HPLC Separation Systems; V. Strategic Considerations behind the HPLC Separations; VI. Specific Physicochemical Considerations on the Individual Chromatographic Modes; VII. The Effect of Temperature and the Thermodynamics of Polypeptide- or Protein-Ligate Interactions; VIII. Factors That Control Performance and Efficiency
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IX. Scaling-Up Possibilities: Heuristic Approaches and Productivity ConsiderationsX. Effect of Mass Transfer Resistances in Preparative HPLC of Polypeptides and Proteins; XI. Summary; References; Chapter 4. Capillary Electrophoresis of Compounds of Biological Interest; I. Introduction; II. Capillary Zone Electrophoresis; III. Migration Behavior of Peptides and Proteins; IV. Modifications of Fused Silica Capillaries; V. Effect of Temperature on Separations; VI. Strategy for Protein Separations; VII. Capillary Gel Electrophoresis; VIII. Micellar Electrokinetic Chromatography
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IX. Capillary ElectrochromatographyX. Applications; References; Chapter 5. Isoelectric Focusing; I. Introduction; II. The Principles of Isoelectric Focusing; III. Analytical Isoelectric Focusing; IV. Two-Dimensional Gel Electrophoresis; V. Preparative Isoelectric Focusing; VI. Capillary Isoelectric Focusing; VII. Summary; VIII. Appendix; References; Chapter 6. Mass Spectrometry of Biomolecules; I. Introduction; II. Examples of Applications of Mass Spectrometry to Biological Research; III. Conclusions; References; Chapter 7. Liquid-Liquid Partitioning Methods for Bioseparations
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I. IntroductionII. Solvent Extraction for Bioseparations; III. Aqueous Two-Phase Partitioning for Bioseparations; IV. Summary; References; Chapter 8. Separation of Nucleic Acids and Proteins; I. Introduction; II. Precipitation of Nucleic Acids; III. Nuclease Treatment; IV. Aqueous Two-Phase Extraction; V. A Case Study; VI. Conclusions; References; Chapter 9. Bioseparations by Displacement Chromatography; I. Introduction; II. Purification of Amino Acids and Peptides by Displacement Chromatography; III. Purification of Proteins by Displacement Chromatography
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IV. Alternative Modes of Displacement Chromatography
,
English
Weitere Ausg.:
ISBN 0-12-045540-4
Sprache:
Englisch
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