UID:
almafu_9958076130902883
Format:
1 online resource (295 p.)
ISBN:
1-281-03270-0
,
9786611032708
,
0-08-053416-3
Content:
HPLC and CE: Principles and Practice presents the latest information on the most powerful separation techniques available: high-performance liquid chromatography (HPLC) and capillary electrophoresis (CE). Fundamental theory, instrumentation, modes of operation, and optimization of separations are presented in a concise, non-technical style to help the user in choosing the appropriate technique quickly and accurately. Well- illustrated and containing convenientend-of-chapter summaries of the major concepts, the book provides in-depth coverage of trouble-shooting, improvement of resolutio
Note:
Description based upon print version of record.
,
Front Cover; HPLC and CE: Principles and Practice; Copyright Page; Contents; Preface; Chapter 1. High-Performance Liquid Chromatography; 1.1 Introduction; 1.2 Classification of Liquid Chromatographic Methods; 1.3 Basic Concepts of HPLC; 1.4 Summary of Major Concepts; References; Chapter 2. Separations in High-Performance Liquid Chromatography; 2.1 Introduction; 2.2 Normal-Phase Chromatography; 2.3 Reversed-Phase Chromatography; 2.4 Hydrophobic-Interaction Chromatography; 2.5 Ion-Exchange Chromatography; 2.6 Size-Exclusion Chromatography; 2.7 Affinity Chromatography; 2.8 Chiral Separations
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2.9 Summary of Major ConceptsReferences; Chapter 3. Instrumentation for High-Performance Liquid Chromatography; 3.1 Introduction; 3.2 Solvent Delivery Systems; 3.3 Solvent Degassing; 3.4 Isocratic versus Gradient Pumping Systems; 3.5 Sample Introduction; 3.6 Columns; 3.7 Detectors; 3.8 Sample Preparation; 3.9 Troubleshooting; 3.10 Summary of Major Concepts; References; Chapter 4. Capillary Electrophoresis; 4.1 Introduction; 4.2 Classification of Electrophoretic Modes; 4.3 Basic Concepts of Capillary Electrophoresis; 4.4 Summary of Major Concepts; References
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Chapter 5. Separation in Capillary Electrophoresis5.1 Introduction; 5.2 Capillary Zone Electrophoresis; 5.3 Micellar Electrokinetic Capillary Chromatography; 5.4 Capillary Gel Electrophoresis; 5.5 Capillary Electrochromatography; 5.6 Chiral Capillary Electrophoresis; 5.7 Capillary Isoelectric Focusing; 5.8 Capillary Isotachophoresis; 5.9 Summary of Major Concepts; References; Chapter 6. Instrumentation of Capillary Electrophoresis; 6.1 Introduction; 6.2 High-Voltage Power Supply; 6.3 Sample Injection; 6.4 Capillaries; 6.5 Detection; 6.6 Fraction Collection; 6.7 Sample Preparation
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6.8 Troubleshooting6.9 Summary of Major Concepts; References; Chapter 7. Data Manipulation; 7.1 Introduction; 7.2 Identification of Peaks; 7.3 Quantitation; 7.4 Summary of Major Concepts; References; Chapter 8. Miniaturization; 8.1 Introduction; 8.2 Classification of Columns; 8.3 Theoretical and Practical Considerations; 8.4 Applications of Miniaturized Liquid Chromatography; 8.5 Separations on a Chip; 8.6 Summary of Major Concepts; References; Index
,
English
Additional Edition:
ISBN 0-12-136640-5
Language:
English
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