UID:
almafu_9958080595302883
Umfang:
1 online resource (379 p.)
ISBN:
1-281-04632-9
,
9786611046323
,
0-08-053997-1
Inhalt:
The design and production of novel peptides and proteins occupy pivotal positions in science and technology and will continue to do so in the 21st century. Protein Engineering and Design outlines the rapid advances in computer-based modeling, protein engineering, and methods needed for protein and peptide preparation and characterization. This indispensable reference lays the groundwork for understanding this multidisciplinary activity while providing an introduction for researchers and students to the field of protein design.Key Features* Introduces and defines the techniqu
Anmerkung:
Description based upon print version of record.
,
Front Cover; Protein Engineering and Design; Copyright Page; Contents; Contributors; Preface; Part I: PREDICTION; Chapter 1. Strategies for the Design of Novel Proteins; I. Introduction; II. Strategies for the Design of Structure; III. Strategies for the Design of Function; IV. Conclusion; References; Chapter 2. Computer Methods in Protein Modeling: An Overview; I. Introduction; II. Early Methods of Computer Modeling ""by Hand""; III. Hardware and Computational Workstyles; IV. Molecular Mechanics, a Controversial Paradigm of Protein Folding; V. What Do We Know about Folding?
,
VI. Electrostatics of Protein StructuresVII. De Novo Design of Proteins: Folding in the Computer; VIII. Secondary Structure Prediction; IX. Surfaces and Volumes; X. Molecular Dynamics Simulations; XI. Free Energy Perturbation; XII. Knowledge-Based Approaches to Model Building; XIII. Conformational Searches; XIV. The Free Energy Problem; XV. Future Trends; References; Part II: PRODUCTION; Chapter 3. The Art of Expression: Sites and Strategies for Heterologous Expression; I. Why Escherichia coli?; II. Genetic Considerations in Expression
,
III. Post-translational Manipulations: Form of the Expressed ProteinIV. Sites of Expression; V. Conclusion; References; Chapter 4. Methods for Expressing Recombinant Proteins in Yeast; I. Introduction; II. Materials and Methodology for Protein Expression in Saccharomyces cerevisiae; III. Summary; References; Chapter 5. In Vitro Mutagenesis; I. Introduction; II. In Vitro Mutagenesis: Principle and Variations; Ill. Examples of Applications; IV. Conclusions; Appendix: Some Key Literature References and Commercial Products for Those Wishing to Use in Vitro Site-Directed Mutagenesis; References
,
Part III: CHARACTERIZATIONChapter 6. Determination of Structures of Larger Proteins in Solution by Three- and Four-Dimensional Heteronuclear Magnetic Resonance Spectroscopy; I. Introduction; II. The Nature of the Structural Data Derived from NMR Measurements; III. General Principles Involved in Deriving Experimental Restraints from NMR Data; IV. Basics of 3D and 4D NMR; V. Heteronuclear 3D and 4D NMR in Practice; VI. Application of 3D and 4D NMR to the Determination of Larger Protein Structures; VII. Concluding Remarks; References; Chapter 7. A Consumer's Guide to Protein Crystallography
,
I. CrystallizationII. Data Collection; III. Phase Determination; IV. Electron Density Map Interpretation; V. Refinement; VI. Exploiting the Structure; VII. Some Final Comments; Appendix: Some Useful Articles and Addresses; References; Chapter 8. Spectroscopic and Calorimetric Methods for Characterizing Proteins and Peptides; I. Introduction; II. Circular Dichroism; III. Fourier-Transform Infrared Spectroscopy; IV. Raman and Resonance Raman Spectroscopies; V. Differential Scanning Calorimetry; References; Part IV: APPLICATIONS
,
Chapter 9. The Design of Polymeric Biomaterials from Natural a-L-Amino Acids
,
English
Weitere Ausg.:
ISBN 0-12-159640-0
Sprache:
Englisch
Bookmarklink
