Umfang:
Online-Ressource (XXIX, 926 S.)
Ausgabe:
Online-Ausg.
ISBN:
9780121820725
Serie:
Biomembranes / ed. by Sidney Fleischer ... Pt. R
Inhalt:
The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences
Anmerkung:
Includes bibliographical references and index
,
Gentle and fast transmembrane reconstitution of membrane proteins
,
Separation of cells and cell organelles by partition in aqueous polymer two-phase systems
,
Transport machinery: An overview
,
Gentle and fast transmembrane reconstitution of membrane proteins
,
Separation of cells and cell organelles by partition in aqueous polymer two-phase systems
,
Transport machinery: An overview
,
Ion carriers in planar bilayers: Relaxation techniques and noise analysis
,
Ion interactions at membranous polypeptide sites using nuclear magnetic resonance: Determining rate and binding constants and site locations
,
Measuring electrostatic potentials adjacent to membranes
,
Determination of surface potential of biological membranes
,
Lipid coumarin dye as a probe of interfacial electrical potential in biomembranes
,
Modulation of membrane protein function by surface potential
,
Use of nonequilibrium thermodynamics in the analysis of transport: General flow-force relationships and the linear domain
,
Cell isolation techniques: Use of enzymes and chelators
,
Cell separation by gradient centrifugation methods
,
Cell separation by elutriation: Major and minor cell types from complex tissues
,
Cell separation using velocity sedimentation at unit gravity and buoyant density centrifugation
,
Separation of cell populations by free-flow electrophoresis
,
Flow cytometry: Rapid isolation and analysis of single cells
,
Use of cell-specific monoclonal antibodies to isolate renal epithelia
,
Pancreatic acini as second messenger models in exocrine secretion
,
Ascites cell preparation: Strains, caveats
,
Direct current electrical measurement in epithelia: Steady-state and transient analysis
,
Impedance analysis in tight epithelia
,
Electrical impedance analysis of leaky epithelia: Theory, techniques, and leak artifact problems
,
Patch-clamp experiments in epithelia: Activation by hormones or neurotransmitters
,
Ionic permeation mechanisms in epithelia: Biionic potentials, dilution potentials, conductances, and streaming potentials
,
Use of ionophores in epithelia: Characterizing membrane properties
,
Cultures as epithelial models: Porous-bottom culture dishes for studying transport and differentiation
,
Volume regulation in epithelia: Experimental approaches
,
Scanning electrode localization of transport pathways in epithelial tissues
,
Experimental control of intracellular environment
,
Implantation of Isolated Carriers and receptors into living cells by Sendai virus envelope-mediated fusion
,
Resonance energy transfer microscopy: Visual colocalization of fluorescent lipid probes in liposomes
,
Permeabilizing mammalian cells to macromolecules
,
Survey of carrier methodology: Strategy for identification, isolation, and characterization of transport systems
,
Kinetics of transport: Analyzing, testing, and characterizing models using kinetic approaches
,
Kinetics of ion movement mediated by carriers and channels
,
Inhibition kinetics of carrier systems
,
Design of simple devices to measure solute fluxes and binding in monolayer cell cultures
,
Utilization of binding energy and coupling rules for active transport and other coupled vectorial processes
,
Generation of steady-state rate equations for enzyme and carrier-transport mechanisms: A microcomputer program
,
Liposome preparation and size characterization
,
Preparation of microcapsules from human erythrocytes: Use in transport experiments of glutathione and its S-conjugate
,
Planar lipid-protein membranes: Strategies of formation and of detecting dependencies of ion transport functions on membrane conditions
,
Spontaneous insertion of integral membrane proteins into preformed unilamellar vesicles
,
Ion carriers in planar bilayers: Relaxation techniques and noise analysis
,
Ion interactions at membranous polypeptide sites using nuclear magnetic resonance: Determining rate and binding constants and site locations
,
Measuring electrostatic potentials adjacent to membranes
,
Determination of surface potential of biological membranes
,
Lipid coumarin dye as a probe of interfacial electrical potential in biomembranes
,
Modulation of membrane protein function by surface potential
,
Use of nonequilibrium thermodynamics in the analysis of transport: General flow-force relationships and the linear domain
,
Cell isolation techniques: Use of enzymes and chelators
,
Cell separation by gradient centrifugation methods
,
Cell separation by elutriation: Major and minor cell types from complex tissues
,
Cell separation using velocity sedimentation at unit gravity and buoyant density centrifugation
,
Separation of cell populations by free-flow electrophoresis
,
Flow cytometry: Rapid isolation and analysis of single cells
,
Use of cell-specific monoclonal antibodies to isolate renal epithelia
,
Pancreatic acini as second messenger models in exocrine secretion
,
Ascites cell preparation: Strains, caveats
,
Direct current electrical measurement in epithelia: Steady-state and transient analysis
,
Impedance analysis in tight epithelia
,
Electrical impedance analysis of leaky epithelia: Theory, techniques, and leak artifact problems
,
Patch-clamp experiments in epithelia: Activation by hormones or neurotransmitters
,
Ionic permeation mechanisms in epithelia: Biionic potentials, dilution potentials, conductances, and streaming potentials
,
Use of ionophores in epithelia: Characterizing membrane properties
,
Cultures as epithelial models: Porous-bottom culture dishes for studying transport and differentiation
,
Volume regulation in epithelia: Experimental approaches
,
Scanning electrode localization of transport pathways in epithelial tissues
,
Experimental control of intracellular environment
,
Implantation of Isolated Carriers and receptors into living cells by Sendai virus envelope-mediated fusion
,
Resonance energy transfer microscopy: Visual colocalization of fluorescent lipid probes in liposomes
,
Permeabilizing mammalian cells to macromolecules
,
Survey of carrier methodology: Strategy for identification, isolation, and characterization of transport systems
,
Kinetics of transport: Analyzing, testing, and characterizing models using kinetic approaches
,
Kinetics of ion movement mediated by carriers and channels
,
Inhibition kinetics of carrier systems
,
Design of simple devices to measure solute fluxes and binding in monolayer cell cultures
,
Utilization of binding energy and coupling rules for active transport and other coupled vectorial processes
,
Generation of steady-state rate equations for enzyme and carrier-transport mechanisms: A microcomputer program
,
Liposome preparation and size characterization
,
Preparation of microcapsules from human erythrocytes: Use in transport experiments of glutathione and its S-conjugate
,
Planar lipid-protein membranes: Strategies of formation and of detecting dependencies of ion transport functions on membrane conditions
,
Spontaneous insertion of integral membrane proteins into preformed unilamellar vesicles
Weitere Ausg.:
Erscheint auch als Druck-Ausgabe Biomembranes ; Pt. R: Transport theory San Diego, Calif. [u.a.] : Academic Press, 1989 ISBN 0121820726
Sprache:
Englisch
Fachgebiete:
Biologie
Bookmarklink
