Format:
Online-Ressource (XLII, 789 S.)
Edition:
Online-Ausg. 2011 Electronic reproduction; Available via World Wide Web
ISBN:
9780121822736
Series Statement:
Methods in enzymology 370
Content:
RNA polymerase is molecule important to gene transcription. Along with associated factors, RNA polymerase is part of the process in which RNA is transcribed to produce a protein. * Construction and purification of RNA polymerases * DNA microarrays and bacterial gene expression * Functional analysis of transcription factors
Content:
RNA polymerase is molecule important to gene transcription. Along with associated factors, RNA polymerase is part of the process in which RNA is transcribed to produce a protein.* Construction and purification of RNA polymerases* DNA microarrays and bacterial gene expression* Functional analysis of transcription factors
Note:
Includes bibliographical references and indexes
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Front Cover; RNA Polymerases and Associated Factors; Copyright Page; Table of Contents; Contributors to Volume 370; Preface; Volumes in Series; Section I: RNA Polymerase Structure and Properties; Chapter 1. Construction, Purification, and Characterization of Escherichia coli RNA Polymerases Tagged with Different Fluorescent Proteins; Chapter 2. Purification of Bacillus subtilis Polymerase and Associated Factors; Chapter 3. Determination of Escherichia coli RNA Polymerase Structure by Single Particle Cryoelectron Microscopy
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Chapter 4. Crystallographic Analysis of Thermus aquaticus RNA Polymerase Holoenzyme and a Holoenzyme/Promoter DNA ComplexChapter 5. Purification of Rhodobacter sphaeroides RNA Polymerase and Its Sigma Factors; Chapter 6. In Vitro Transcription Assays Using Components from Methanothermobacter thermautotrophicus; Chapter 7. Isolation and Characterization of Streptomyces coelicolor RNA Polymerase, Its Sigma, and Antisigma Factors; Chapter 8. Bacteriophage N4-Coded, Virion- Encapsulated DNA-Dependent RNA Polymerase
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Chapter 9. Preparation and Characterization of Recombinant Thermus aquatics RNA PolymeraseChapter 10. Purification and Assay of Upstream Activation Factor, Core Factor, Rrn3p, and Yeast RNA Polymerase I; Chapter 11. Purification and Transcriptional Analysis of RNA Polymerase I Holoenzymes from Broccoli (Brassica oleracea) and Frog (Xenopus laevis); Chapter 12. Assays and Affinity Purification of Biotinylated and Nonbiotinylated Forms of Double-Tagged Core RNA Polymerase II from Saccharomyces cerevisiae; Chapter 13. Dephosphorylation of the Carboxyl-Terminal Domain of RNA Polymerase II
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Chapter 14. RNA Polymerase III from the Fission Yeast, Schizosaccharomyces pombeChapter 15. Purification of Highly-Active and Soluble Escherichia coli 70 Polypeptide Overproduced at Low Temperature; Chapter 16. Expression, Purification of, and Monoclonal Antibodies to Factors from Escherichia coli; Chapter 17. Studying Sigma-Core Interactions in Escherichia coli RNA Polymerase by Electrophoretic Shift Assays and Luminescence Resonance Energy Transfer; Chapter 18. Assay of Escherichia coli RNA Polymerase: Sigma-Core Interactions
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Chapter 19. Purification and Characterization of Bacteriophage-Encoded Inhibitors of Host RNA Polymerase: T-Odd Phage gp2-like ProteinsSection II: Analyses of Promoter and Transcription Patterns; Chapter 20. A Procedure for Identifying Loosely Conserved Protein-Binding DNA Sequences; Chapter 21. Computational Detection of Vertebrate RNA Polymerase II Promoters; Chapter 22. Detection of DNA-Binding Helix-Turn-Helix Motifs in Proteins Using the Pattern Dictionary Method; Chapter 23. DNA Microarrays and Bacterial Gene Expression
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Chapter 24. Analysis of Microarray Data for the marA, soxS, and rob Regulons of Escherichia coli
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Literaturangaben
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Electronic reproduction; Available via World Wide Web
In:
Pt. C
Additional Edition:
Erscheint auch als Druck-Ausgabe RNA polymerases and associated factors ; Pt. C Amsterdam [u.a.] : Acad. Press, 2003 ISBN 0121822737
Language:
English
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