Umfang:
Online-Ressource (XXXV, 582 S.)
Ausgabe:
Online-Ausg. 2009 Electronic reproduction; Available via World Wide Web
ISBN:
0121828131
,
9780121828134
Serie:
Methods in enzymology 408
Inhalt:
This volume provides detailed coverage of modern methods for molecular analysis of enzymes and enzyme systems that function in the maintenance of genome integrity. Coverage areas include base excision repair, nucleotide excision repair, translesion DNA polymerases, mismatch repair, genetic recombination, and double strand break repair. *A laboratory standard for more than 40 years *Over 400 volumes strong *Also available on ScienceDirect*Part A of a 2-part series
Inhalt:
This volume provides detailed coverage of modern methods for molecular analysis of enzymes and enzyme systems that function in the maintenance of genome integrity. Coverage areas include base excision repair, nucleotide excision repair, translesion DNA polymerases, mismatch repair, genetic recombination, and double strand break repair.*A laboratory standard for more than 40 years*Over 400 volumes strong*Also available on ScienceDirect*Part A of a 2-part series
Anmerkung:
Literaturangaben
,
Cover Page; Table of Contents; Contributors of Volume 408; Volumes in Series; Chapter 1: Assays for Determining Lesion Bypass Efficiency and Mutagenicity of Site-Specific DNA Lesions In Vivo; Introduction; Genome Construction; Genome Normalization; Lesion Bypass (CRAB) Assay; Lesion Mutagenesis (REAP) Assay; Conclusion; References; Chapter 2: Oxidative DNA Glycosylases: Recipes from Cloning to Characterization; Introduction; Purification of DNA Glycosylases; Preparation of Oligonucleotide Substrates; Activity Assays for Oxidative DNA Glycosylases; Acknowledgment; References
,
Chapter 3: Purification and Characterization of NEIL1 and NEIL2, Members of a Distinct Family of Mammalian DNA Glycosylases for Repair of Oxidized BasesEndogenous and Induced DNA Damage; Repair of Oxidized Base Lesions in the Genome; Two Classes of Oxidized Base-Specific Glycosylases; Human Genome Database Analysis and Discovery of MutM/Nei-Like Proteins; Enzymatic Activity of NEIL1 and NEIL2 with Duplex or Bubble Oligonucleotide Substrates; Detection of DNA Glycosylase Activity of Recombinant NEILs by Trapping Analysis; Acknowledgments; References
,
Chapter 4: Analysis of Base Excision DNA Repair of the Oxidative Lesion 2-Deoxyribonolactone and the Formation of DNA-Protein Cross-LinksIntroduction; Preparation of dL-DNA Substrates; Analysis of dL-mediated Spontaneous DNA-Protein Cross-Links; Analysis of dL-Specific BER; Acknowledgments; References; Chapter 5: Isolation and Analyses of MutY Homologs (MYH); Introduction; Isolation of MutY and MYH Proteins; Functional Assays of MutY and MYH; Protein Interaction Assays; Acknowledgments; References
,
Chapter 6: Use of Yeast for Detection of Endogenous Abasic Lesions, Their Source, and Their RepairAP Sites in DNA and Related 3'-Blocked and 5'-Blocked Single Strand Breaks Form an Abundant and Deleterious Class of Endogenous DNA Damage; Endogenous AP Sites Cause Cell Death in the Absence of Apn1, Apn2, and Rad1-Rad10; Use of the apn1 apn2 rad1 Triple Mutant to Study the Origin and Repair of Endogenous AP Sites in DNA; Use of the apn1 apn2 rad14 Triple Mutant to Study the Origin and Repair of Endogenous AP Sites in DNA; Conclusions and Model; Acknowledgments; References
,
Chapter 7: Activities and Mechanism of DNA Polymerase betaIntroduction; Kinetic Characteristics of DNA Synthesis; Measurement of dRP Lyase Activity; Measurement of In Vitro BER; Reagents; Acknowledgment; References; Chapter 8: Direct Removal of Alkylation Damage from DNA by AlkB and Related DNA Dioxygenases; Purification of AlkB, ABH2, and ABH3; 14C-Methylated Substrates; Assays; In Vivo Assay: Survival of Alkylated Single-Stranded DNA Bacteriophage in E. coli AlkB Mutants; References; Chapter 9: Purification and Characterization of DNA Photolyases; Introduction
,
Overexpression and Purification of Photolyases
,
Electronic reproduction; Available via World Wide Web
In:
Pt. A
Weitere Ausg.:
Erscheint auch als Druck-Ausgabe DNA repair ; Pt. A Amsterdam [u.a.] : Elsevier, 2006 ISBN 0121828131
Weitere Ausg.:
ISBN 9780121828134
Sprache:
Englisch
Schlagwort(e):
Aufsatzsammlung
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