Umfang:
1 Online-Ressource (xvii, 342 Seiten)
,
Illustrationen
Ausgabe:
Reproduktion Life Sciences. Biochemistry, Genetics and Molecular Biology Electronic reproduction; Mode of access: World Wide Web
ISBN:
9780080525815
,
9781281743619
Inhalt:
Three-Dimensional Electron Microscopy of Macromolecular Assemblies is the first systematic introduction to single-particle methods of reconstruction. It covers correlation alignment, classification, 3D reconstruction, restoration, and interpretation of the resulting 3D images in macromolecular assemblies. It will be an indispensable resource for newcomers to the field and for all using or adopting these methods. Key Features * Presents methods that offer an alternative to crystallographic techniques for molecules that cannot be crystallized * Describes methods that have been instrumental in exploring the three-dimensional structure of * the nuclear pore complex * the calcium release channel; * the ribosome * chaperonins
Inhalt:
Three-Dimensional Electron Microscopy of Macromolecular Assembliesis the first systematic introduction to single-particle methods of reconstruction. It covers correlation alignment, classification, 3D reconstruction, restoration, and interpretation of the resulting 3D images in macromolecular assemblies. It will be an indispensable resource for newcomers to the field and for all using or adopting these methods. Key Features * Presents methods that offer an alternative to crystallographic techniques for molecules that cannot be crystallized * Describes methods that have been instrumental in exploring the three-dimensional structure of * the nuclear pore complex * the calcium release channel, * the ribosome * chaperonins
Anmerkung:
Includes bibliographical references (p. 293-331) and index
,
Front Cover; Three-Dimensional Electron Microscopy of Macromolecular Assemblies; Copyright Page; Contents; Preface; Chapter 1. Introduction; I. The Electron Microscope and Biology; II. Single-Particle versus Crystallographic Analysis; III. Crystallography without Crystals; IV. Toward a Unified Approach to Structure Research; V. The Electron Microscope and the Computer; Chapter 2. Electron Microscopy of Macromolecular Assemblies; I. Specimen Preparation Methods; II. Principle of Image Formation in the Electron Microscope; III. Special Imaging Techniques; Chapter 3. Two-Dimensional Averaging TechniquesI. Introduction; II. Digitization and Selection of Particles; III. Alignment Methods; IV. Averaging and Global Variance Analysis; V. Resolution; VI. Validation of the Average Image: Rank Sum Analysis; VII. Outlier Rejection: ""Odd Men Out"" Strategy; Chapter 4. Multivariate Statistical Analysis and Classification of Images; I. Introduction; II. Theory of Correspondence Analysis; III. Correspondence Analysis in Practice; IV. Classification; Chapter 5. Three-Dimensional Reconstruction; I. Introduction; II. General Mathematical Principles; III. Rationales of Data Collection: Reconstruction SchemesIV. Overview of Existing Reconstruction Techniques; V. The Random Conical Reconstruction Scheme in Practice; VI. Merging of Reconstructions; VII. Three-Dimensional Restoration; VIII. Angular Refinement Techniques; IX. Transfer Function Correction; Chapter 6. Interpretation of Three Dimensional Images of Macromolecules; I. Preliminaries: Significance, Experimental Validity, and Meaning; II. Assessment of Statistical Significance; III. Validation and Consistency; IV. Visualization and Segmentation; V. Juxtaposition with Existing KnowledgeChapter 7. Example for an Application: Calcium Release Channel; I. Introduction; II. Image Processing and Three Dimensional Reconstruction of the Calcium Release Channel; Appendix 1: Software Implementations; I. Introduction; II. Basic Design Features; Ill. Existing Packages; IV. Interfacing to Other Software; V. Documentation; Appendix 2: Macromolecular Assemblies Reconstructed from Images of Single Macromolecules; Bibliography; Index
,
Electronic reproduction; Mode of access: World Wide Web
Weitere Ausg.:
ISBN 0122650409
Weitere Ausg.:
ISBN 9780122650406
Weitere Ausg.:
Erscheint auch als Druck-Ausgabe Frank, Joachim, 1940 - Three-dimensional electron microscopy of macromolecular assemblies San Diego [u.a.] : Academic Press, 1996 ISBN 0122650409
Sprache:
Englisch
Fachgebiete:
Biologie
Schlagwort(e):
Makromolekül
;
Elektronenmikroskopie
;
Electronic books
Mehr zum Autor:
Frank, Joachim 1940-
Bookmarklink
