UID:
almahu_9948595845702882
Format:
1 online resource (518 p.)
ISBN:
1-282-52611-1
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9786612526114
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0-08-091904-9
Content:
Advances in genomic and proteomic profiling of disease have transformed the field of molecular diagnostics, thus leading the way for a major revolution in clinical practice. While the range of tests for disease detection and staging is rapidly expanding, many physicians lack the knowledge required to determine which tests to order and how to interpret results. This handbook provides a complete guide to the use and interpretation of molecular testing in the clinical arena. No other available resource offers this emphasis, comprehensive scope, and practical utility in the clinical setting.〈b
Note:
Description based upon print version of record.
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Front Cover; Molecular Diagnostics; Copyright Page; Contents; Foreword; PART I: Introduction and Methodology; Chapter 1. Quantitative PCR: An Introduction; INTRODUCTION; THEORETICAL VERSUS ACTUAL RESULTS; SPECIMEN COLLECTION AND SPECIMEN COLLECTION AND; NUCLEIC ACID EXTRACTION; OPTIMIZATION; INHIBITORS; HOT STARTS; DETECTION; REAL-TIME PCR; QUANTITATION; COMPETITIVE PCR; QUANTITATIVE REVERSE-TRANSCRIPTASE PCR; CONCLUSION; REFERENCES; Chapter 2. Signal Amplification Methods in Molecular Diagnostics; INTRODUCTION AND DEFINITIONS; BRANCHED DNA AMPLIFICATION; HYBRID CAPTURE; INVADER TECHNOLOGY
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OTHER TECHNOLOGIESREFERENCES; Chapter 3. The Lab-on-a-Chip Approach for Molecular Diagnostics; INTRODUCTION; ON-CHIP SAMPLE PREPARATION FOR MOLECULAR DIAGNOSTICS; THE LAB-ON-A-CHIP APPROACH FOR GENETIC ANALYSIS; SUMMARY; ACKNOWLEDGEMENTS; REFERENCES; Chapter 4. Nucleic Extraction and Amplification; INTRODUCTION; GENERAL CONSIDERATIONS; STARTING MATERIAL; EXTRACTION METHODS FOR DNA; AMPLIFICATION METHODS; REFERENCES; INTERNET RESOURCES; Chapter 5. General Procedures; I. INTRODUCTION; II. GEL ELECTROPHORESIS; III. NUCLEIC ACID QUANTIFICATION BY UV ABSORBANCE; REFERENCES
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Chapter 6. Next-Generation Sequencing in Molecular DiagnosticsINTRODUCTION; MASSIVELY PARALLEL SEQUENCING PLATFORMS; GENOMIC ISOLATION TECHNIQUES; DATA ANALYSIS AND STORAGE; CLINICAL POTENTIAL AND UTILITY; CHALLENGES FOR TEST REPORTING AND ETHICAL CONSIDERATIONS; REFERENCES; PART II: Molecular Techniques for Anatomic and Surgical Pathology; Chapter 7. In Situ Hybridization: Principles and Applications; I. INTRODUCTION; II. COMPONENTS OF ISH HYBRIDIZATION ASSAYS; III. AUTOMATION; IV. TROUBLESHOOTING; V. APPLICATIONS; REFERENCES; PART III: Quality Assurance, Regulatory, and Ethical Issues
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Chapter 8. Billing and Reimbursement for Molecular DiagnosticsINTRODUCTION; COVERAGE; PAYMENT; SUMMARY; REFERENCES; Chapter 9. Molecular Pathology and Infectious Diseases; I. INTRODUCTION; II. EMERGING INFECTIOUS DISEASES; III. BIOFILMS; IV. MICRORNAS IN LARGE DNA VIRUSES; V. LEISHMANIA; VI. THE FUTURE; REFERENCES; Chapter 10. Ethical Considerations in Molecular Diagnostics; INTRODUCTION; HISTORICAL PERSPECTIVE; PRINCIPLES OF ETHICAL CONDUCT; APPLICATIONS OF ETHICAL PRINCIPLES; CONCLUSIONS; REFERENCES; Chapter 11. Quality Control, Assurance, Identification, and Standards; INTRODUCTION
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BACKGROUNDINDIVIDUAL SAMPLE QC; PLATE OR BATCH QC; ASSAY OR PLATFORM QC; OVERALL LABORATORY AND FACILITIES QC; REFERENCE MATERIALS AND POSITIVE CONTROL SAMPLES; REPORTING AND INFORMATION SYSTEMS QC; PERSONNEL QUALIFICATIONS; PROFICIENCY TESTING; INTERNET RESOURCES; REFERENCES; Chapter 12. Regulatory Issues in Molecular Diagnostics; INTRODUCTION; GENERAL LABORATORY CERTIFICATION; SPECIFIC MOLECULAR DIAGNOSTIC LABORATORY CERTIFICATION AND PROFICIENCY TESTING; SPECIAL OVERSIGHT OF MOLECULAR GENETIC TESTING; THE FDA AND ANALYTE-SPECIFIC REAGENTS; PERSONNEL REQUIREMENTS AND CERTIFICATION
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INTELLECTUAL PROPERTY
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English
Additional Edition:
ISBN 0-12-369428-0
Language:
English
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