UID:
almahu_9949697896502882
Umfang:
1 online resource (391 p.)
ISBN:
1-281-04656-6
,
9786611046569
,
0-08-053854-1
Inhalt:
PCR Strategies expands and updates the landmark volume PCR Protocols. It is a companion laboratory manual that provides a completely new set of up-to-date strategies and protocols for getting the most from PCR.The editors have organized the book into four sections, focusing on principles, analyses, research applications, and alternative strategies for a wide variety of basic and clinical needs. If you own PCR Protocols, you will want PCR Strategies. If you don't own PCR Protocols, you will want to buy both!Key Features* Concepts explained*
Anmerkung:
Description based upon print version of record.
,
Front Cover; PCR STRATEGIES; Copyright Page; CONTENTS; Contributors; Foreword; Preface; Part One: KEY CONCEPTS FOR PCR; Chapter 1. The Use of Cosolvents to Enhance Amplification by the Polymerase Chain Reaction; Chapter 2. DNA Polymerase Fidelity: Misinsertions and Mismatched Extensions; Chapter 3. Extraction of Nucleic Acids: Sample Preparation from Paraffin-Embedded Tissues; Chapter 4. Thermostable DNA Polymerases; Chapter 5. Amplification of RNA: High-Temperature Reverse Transcription and DNA Amplification with Thermus thermophilus DNA Polymerase
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Chapter 6. Nucleic Acid Hybridization and Unconventional BasesChapter 7. Practical Considerations for the Design of Quantitative PCR Assays; Part Two: ANALYSIS OF PCR PRODUCTS; Chapter 8. Carrier Detection of Cystic Fibrosis Mutations Using PCR-Amplified DNA and a Mismatch-Binding Protein, MutS; Chapter 9. Single-Stranded Conformational Polymorphisms; Chapter 10. Analysis of PCR Products by Covalent Reverse Dot Blot Hybridization; Chapter 11. High-Performance Liquid Chromatography Analysis of PCR Products; Chapter 12. Heteroduplex Mobility Assays for Phylogenetic Analysis
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Chapter 13. PCR Amplification of VNTRsPart Three: RESEARCH APPLICATIONS; Chapter 14. Site-Specific Mutagenesis Using the Polymerase Chain Reaction; Chapter 15. Exact Quantification of DNA-RNA Copy Numbers by PCR-TGGE; Chapter 16. The in Situ PCR: Amplication and Detection of DNA in a Cellular Context; Chapter 17. Y Chromosome-Specific PCR: Maternal Blood; Chapter 18. Genomic Subtraction; Chapter 19. DNA Amplification-Restricted Transcription Translation (DARTT): Analysis of in Vitro and in Situ Protein Functions and Intermolecular Assembly
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Chapter 20. DNA and RNA Fingerprinting Using Arbitrarily Primed PCRChapter 21. PCR-Based Screening of Yeast Artificial Chromosome Libraries; Chapter 22. Oligonucleotide Ligands That Discriminate between Theophylline and Caffeine; Chapter 23. Generation of Single-Chain Antibody Fragments by PCR; Chapter 24. Longer PCR Amplifications; Chapter 25. Direct Analysis of Specific Bonds from Arbitrarily Primed PCR Reactions; Part Four: ALTERNATIVE AMPLIFICATION STRATEGIES; Chapter 26. Detection of Leber's Hereditary Optic Neuropathy by Nonradioactive Ligase Chain Reaction
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Chapter 27. Detection of Listeria monocytogenes by PCR-Coupled Ligase Chain ReactionIndex
,
English
Weitere Ausg.:
ISBN 0-12-372182-2
Sprache:
Englisch
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