Format:
Online-Ressource
,
Ill.
Edition:
Online-Ausg.
ISBN:
0123736501
,
9780123736505
Series Statement:
Methods in enzymology 419
Content:
This is the second of three planned volumes in the Methods in Enzymology series on the topic of stem cells. This volume is a unique anthology of stem cell techniques focusing on adult stem cells, and written by experts from the top laboratories in the world. The contributors not only have hands-on experience in the field but often have developed the original approaches that they share with great attention to detail. The chapters provide a brief review of each field followed by a "cookbook" and handy illustrations. The collection of protocols includes the isolation and maintenance of stem cells from various species using "conventional" and novel methods, such as derivation of ES cells from single blastomeres, differentiation of stem cells into specific tissue types, isolation and maintenance of somatic stem cells, stem cell-specific techniques and approaches to tissue engineering using stem cell derivatives. The reader will find that some of the topics are covered by more than one group of authors and complement each other. Comprehensive step-by-step protocols and informative illustrations can be easily followed by even the least experienced researchers in the field, and allow the setup and troubleshooting of these state-of-the-art technologies in other laboratories. * Provides complete coverage spanning from derivation/isolation of stem cells, and including differentiation protocols, characterization and maintenance of derivatives and tissue engineering * Presents the latest most innovative technologies * Addresses therapeutic relevance including FDA compliance and tissue engineering
Content:
EctodermChapter 1: Neural Stem cell isolation and characterization -- Reagents and Instrumentation -- Methods -- Chapter 2: Neural stem cells and their manipulation -- Adult niches for stem cells In vivo -- In vitro manipulation of NSCs -- Sample protocols for the culture and characterization of NSCs -- Chapter 3: Retinal stem cells -- Materials and methods -- Chapter 4: Epithelial skin stem cells -- Introduction to skin epithelial stem cells -- Epithelial skin organization and the hair cycle -- Characteristics of skin epithelial stem cells -- Chapter 5: Dental pulp stem cells -- Identification of dental pulp stem cells -- Isolation of DPSCs -- Differentiation of DPSCs -- Section II: Mesoderm -- Chapter 6: Postnatal skeletal stem cells -- Introduction -- Skeletal stem cells and mesenchymal stem cells -- Defining a skeletal ttem cell -- Origin and nature of skeletal stem cells -- Plasticity of BMSCs -- Role of skeletal stem cells in disease -- Molecular engineering -- Potential use of skeletal stem cells in tissue engineering and regenerative Medicine -- Establishment of nonclonal populations of BMSCs in vitro -- Establishment of clonal populations of BMSCs in vitro -- Determination of colony-forming efficiency -- In vitro differentiation of BMSCs -- In vivo differentiation of BMSCs -- Chapter 7: Hematopoietic stem Cells -- Identification and enrichment of HSCs -- Functional characterization of candidate HSCs -- Long-term culture of candidate HSCs and progenitors -- Chapter 8: Hemangioblasts and their progeny -- Ex vivo expansion of CD133+ cells -- Hematopoietic and endothelial differentiation of single cell-derived EGFP+ clones -- Phenotypical and functional studies of CD133-derived cells during ex vivo expansion and differentiation -- Materials -- Reagents -- Methods -- Chapter 9: Kidney epithelial cells -- Analysis of steady state tubular epithelium maintenance -- Regeneration of experimentally injured kidney tubules -- Extratubular progenitor cells -- Culture of human tubular progenitor cells -- Chapter 10: Ovarian germ cells -- Origin of primordial germ cells -- Formation of human fetal oocytes and primordial and primary follicles -- Immune system-related cells and oogenesis in fetal ovaries -- Fetal programming of follicular renewal during adulthood -- "Ovary within the ovary " pattern and Thy-1 differentiation protein -- Cessation of adult oogenesis -- Oogenesis and follicular renewal in adult ovaries -- Working hypothesis on the role of the gonadal environment -- Differentiation of stem cells and tissue control system theory -- Potential treatment of ovarian infertility by producing new primary follicles In vivo -- Mystery of the origin of oocytes in adult mammalian ovaries.
Note:
Includes bibliographical references and indexes. - Print version record
Additional Edition:
Erscheint auch als Druck-Ausgabe Adult stem cells Amsterdam : Elsevier, Academic Press, 2006 ISBN 0123736501
Additional Edition:
ISBN 9780123736505
Language:
English
Subjects:
Biology
Keywords:
Adulte Stammzelle
;
Adulte Stammzelle
;
Electronic books
;
Aufsatzsammlung
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