Umfang:
Online Ressource (xv, 472 p.)
ISBN:
9780123947956
,
0123947952
,
1299619185
,
9781299619180
,
9780123944467
Inhalt:
Proteomic and Metabolomic Approaches to Biomarker Discovery demonstrates how to leverage biomarkers to improve accuracy and reduce errors in research. Disease biomarker discovery is one of the most vibrant and important areas of research today, as the identification of reliable biomarkers has an enormous impact on disease diagnosis, selection of treatment regimens, and therapeutic monitoring. Various techniques are used in the biomarker discovery process, including techniques used in proteomics, the study of the proteins that make up an organism, and metabolomics, the study of chemical fingerprints created from cellular processes. Proteomic and Metabolomic Approaches to Biomarker Discovery is the only publication that covers techniques from both proteomics and metabolomics and includes all steps involved in biomarker discovery, from study design to study execution. The book describes methods, and presents a standard operating procedure for sample selection, preparation, and storage, as well as data analysis and modeling. This new standard effectively eliminates the differing methodologies used in studies and creates a unified approach. Readers will learn the advantages and disadvantages of the various techniques discussed, as well as potential difficulties inherent to all steps in the biomarker discovery process. A vital resource for biochemists, biologists, analytical chemists, bioanalytical chemists, clinical and medical technicians, researchers in pharmaceuticals, and graduate students, Proteomic and Metabolomic Approaches to Biomarker Discovery provides the information needed to reduce clinical error in the execution of research. Describes the use of biomarkers to reduce clinical errors in research. Includes techniques from a range of biomarker discoveries. Covers all steps involved in biomarker discovery, from study design to study execution
Anmerkung:
Includes bibliographical references and index. - Description based on print version record
,
Front Cover; Proteomic and Metabolomic Approaches to Biomarker Discovery; Copyright; Contents; Preface; List of Contributors; Chapter 1 - Biomarker Discovery: Study Design and Execution; ABBREVIATIONS; INTRODUCTION; DEFINITIONS; THE CURRENT STATE OF BIOMARKER DISCOVERY; STUDY DESIGN AND EXECUTION; ERRORS IN STUDY DESIGN; ERRORS IN STUDY EXECUTION; SPECIFICITY OF PROTEINS AS BIOMARKERS; STATISTICAL DATA ANALYSIS; RECOMMENDATIONS; CONCLUDING REMARKS AND RECOMMENDATIONS; Acknowledgments; References; Chapter 2 - Proteomic and Mass Spectrometry Technologies for Biomarker Discovery; INTRODUCTION
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PROTEIN BIOMARKER DISCOVERY AND DEVELOPMENT PIPELINEPROTEOMIC SAMPLES; PROTEIN IDENTIFICATION BY MASS SPECTROMETRY; POST-TRANSLATIONAL MODIFICATIONS AS DISEASE BIOMARKERS; PROTEIN QUANTIFICATION BY MASS SPECTROMETRY; BIOMARKER VERIFICATION; BIOMARKER VALIDATION; LIMITATIONS OF MASS SPECTROMETRY FOR PROTEIN BIOMARKER DISCOVERY; CONCLUSIONS AND FUTURE OUTLOOK: INTEGRATED BIOMARKER DISCOVERY PLATFORM; References; Chapter 3 - Tissue Sample Preparation for Proteomic Analysis; INTRODUCTION; TYPES OF TISSUES AVAILABLE FOR MS-BASED PROTEOMICS; TISSUE DISRUPTION/HOMOGENIZATION
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EXTRACTION/SOLUBILIZATION BUFFERSCONCLUDING REMARKS; Acknowledgment; References; Chapter 4 - Sample Preparation in Global Metabolomics of Biological Fluids and Tissues; ABBREVIATIONS; INTRODUCTION; AN IDEAL SAMPLE PREPARATION METHOD FOR GLOBAL METABOLOMICS?; SAMPLE PREPARATION METHODS FOR BIOFLUIDS; TISSUE METABOLOMICS; NEW TRENDS IN SAMPLE PREPARATION FOR GLOBAL METABOLOMICS; CONCLUSIONS AND FUTURE PERSPECTIVE; Acknowledgment; References; Chapter 5 - Serum and Plasma Collection: Preanalytical Variables and Standard Operating Procedures in Biomarker Research; INTRODUCTION
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IMPORTANCE OF PREANALYTICAL VARIABLESSTANDARD OPERATING PROCEDURES (SOPS); SAMPLE SELECTION CONSIDERATIONS; HUMAN BLOOD AND ITS COMPONENTS; OTHER BIOSAMPLES; BLOOD-BORNE PATHOGENS, UNIVERSAL PRECAUTIONS, AND SAFETY; HUMAN SUBJECT RESEARCH PROTECTIONS; CONCLUSIONS; References; Chapter 6 - Current NMR Strategies for Biomarker Discovery; INTRODUCTION: WHY NMR?; NMR HARDWARE ADVANCEMENT; SAMPLE PREPARATION FOR NMR ANALYSIS; ONE-DIMENSIONAL NMR METHODS: 1H, 13C, 31P; 2D METHODS; TARGETED METABOLIC PROFILING; HIGH-RESOLUTION MAGIC ANGLE SPINNING (HR-MAS) NMR SPECTROSCOPY
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MAGNETIC RESONANCE SPECTROSCOPY (MRS)NMR DATA PROCESSING AND PREPARATION FOR STATISTICAL ANALYSIS; NMR METABOLITE IDENTIFICATION; FUTURE DIRECTIONS AND CONCLUSION; References; Chapter 7 - Using Data-Independent Mass Spectrometry to Extend Detectable Dynamic Range without Prior Fractionation; INTRODUCTION; PACIFIC AND QUANTIFICATION; PROTEOME PROFILING WITH PACIFIC; PERSPECTIVES; References; Chapter 8 - Gas Chromatography/Mass Spectrometry-Based Metabonomics; INTRODUCTION; GC/MS IN METABONOMICS; STRATEGIES TO ADDRESS LARGE-SCALE METABONOMIC INVESTIGATIONS; CONCLUSION AND FUTURE OUTLOOK
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References
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Machine generated contents note: Abbreviations
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Definitions
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The Current State of Biomarker Discovery
,
Study Design and Execution
,
Errors in Study Design
,
Errors in Study Execution
,
Specificity of Proteins as Biomarkers
,
Statistical Data Analysis
,
Recommendations
,
Concluding Remarks and Recommendations
,
Acknowledgments
,
References
,
Introduction
,
Protein Biomarker Discovery and Development Pipeline
,
Proteomic Samples
,
Protein Identification by Mass Spectrometry
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Post-Translational Modifications as Disease Biomarkers
,
Protein Quantification by Mass Spectrometry
,
Biomarker Verification
,
Biomarker Validation
,
Limitations of Mass Spectrometry for Protein Biomarker Discovery
,
Conclusions and Future Outlook: Integrated Biomarker Discovery Platform
,
References
,
Introduction
,
Types of Tissues Available for MS-Based Proteomics
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Tissue Disruption/Homogenization
,
Extraction/Solubilization Buffers
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Concluding Remarks
,
Acknowledgments
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References
,
Abbreviations
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Introduction
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An Ideal Sample Preparation Method for Global Metabolomics?
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Sample Preparation Methods for Biofluids
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Tissue Metabolomics
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New Trends in Sample Preparation for Global Metabolomics
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Conclusions and Future Perspective
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Acknowledgments
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References
,
Introduction
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Importance of Preanalytical Variables
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Standard Operating Procedures (SOPs)
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Sample Selection Considerations
,
Human Blood and Its Components
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Other Biosamples
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Blood-Borne Pathogens, Universal Precautions, and Safety
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Human Subjects Research Protections
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Conclusions
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References
,
Introduction: Why NMR?
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NMR Hardware Advancement
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Sample Preparation for NMR Analysis
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One-Dimensional NMR Methods: 1H, 13C, 31P
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2D Methods
,
Targeted Metabolic Profiling
,
High-Resolution Magic Angle Spinning (HR-MAS) NMR Spectroscopy
,
Magnetic Resonance Spectroscopy (MRS)
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NMR Data Processing and Preparation for Statistical Analysis
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NMR Metabolite Identification
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Future Directions and Conclusion
,
References
,
Introduction
,
PAcIFIC and Quantification
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Proteome Profiling with PAcIFIC
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Perspectives
,
References
,
Introduction
,
GC/MS in Metabonomics
,
Strategies to Address Large-Scale Metabonomic Investigations
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Conclusion and Future Outlook
,
References
,
Introduction
,
Chromatographic Methods for Metabolite Profiling
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Detection
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Quality Control, Data Analysis, and Biomarker Detection
,
Metabolite Identification and Biomarker Validation
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Conclusions
,
References
,
Analysis of Metabolite Profiles Using Capillary Electrophoresis-Mass Spectrometry
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Analysis of Protein Expression Levels Using Capillary Electrophoresis-Mass Spectrometry
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Conclusion
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Acknowledgments
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References
,
Introduction
,
The Evolution of 2-DE Toward Proteomics Applications
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Low-Abundance Proteins Are Not Resolved by 2-DE Alone
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Enhancing Low-Abundance Proteins
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The Discovery of Protein Markers with 2-DE and Its Association with Low-Abundance Protein Enrichment
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Conclusions
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Dedication
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Acknowledgments
,
References
,
Introduction
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Gel Electrophoresis: Historical Perspective
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Two-Dimensional Differential In-Gel Electrophoresis
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Strengths and Weaknesses of 2D-PAGE and 2D-DIGE
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Application of 2D-DIGE to Biomarker Discovery
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Conclusions
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Acknowledgments
,
References
,
Introduction
,
Affinity Targeting Methods
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Single Gene Expression Product Selection
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Post-Translational Modifications
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Abundant Protein Removal
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Conclusions
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References
,
Introduction
,
Aspartate-Selective Acid Proteolysis
,
Microwave-Supported Asp-Selective Acid Proteolysis
,
Methods Development with Microwave-Supported Acid Hydrolysis
,
Applications of Microwave-Supported Acid Hydrolysis
,
Advantages and Disadvantages of Microwave-Supported Asp-Selective Acid Proteolysis
,
References
,
Introduction
,
Depletion
,
Fractionation Procedures for Proteins and Metabolites
,
Affinity Chromatography
,
Isoelectric Focusing
,
Size Exclusion Chromatography
,
Conclusions
,
References
,
Protein Identification
,
Metabolite Identification in Global Metabolomics
,
References
,
Introduction
Weitere Ausg.:
ISBN 0123944465
Weitere Ausg.:
Erscheint auch als Druck-Ausgabe Proteomic and metabolomic approaches to biomarker discovery Amsterdam [u.a.] : Elsevier/AP, Academic Press, 2013 ISBN 9780123944467
Sprache:
Englisch
Fachgebiete:
Biologie
Schlagwort(e):
Proteomanalyse
;
Metabolom
;
Biomarker
;
Electronic books
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