UID:
almafu_9960073105602883
Umfang:
1 online resource (vii, 289 pages) :
,
illustrations (some color)
ISBN:
9780124160019
,
0124160018
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9781299137271
,
129913727X
Serie:
Gale eBooks
Inhalt:
In Situ Molecular Pathology and Co-Expression Analyses explains, in easy-to-understand language, simplified ways of understanding and performing in situ hybridization and immunohistochemistry tests. The book also focuses on straightforward protocols used to simultaneously detect two or more proteins/nucleic acids within intact tissue by doing co-expression analyses. The fields of in situ hybridization and immunohistochemistry have expanded rapidly due to the use of computer-based analysis. To get the most out of these automated platforms, researchers and diagnostic biomedical
Anmerkung:
Description based upon print version of record.
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Front Cover; In Situ Molecular Pathology and Co-Expression Analyses; Copyright Page; Contents; Acknowledgments; 1 Introduction; Suggested Readings; 2 The Basics of Molecular Pathology; Introduction; The Structure of DNA and RNA; The Basic Units of DNA and RNA; Homology; Key Terms in Molecular Biology; The Hydrogen Bond; The "Breathability" of the Hybridized Complexes; Denaturing and Annealing: Signal and Background; The Melting Temperature Curve; Probe Concentration and Probe Size
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The Hybridization Signal with in Situ Hybridization is More Than the Melting Curve: The Effect of Target Copy NumberThe Concentration of Salt in the Stringent Wash; The Structure of Proteins; The Hybridization of a Primary Antibody and Its Epitope; Denaturing Protein-Protein Complexes; Detection Systems for Nucleic Acid-Nucleic Acid and Protein-Protein Hybrids; The Biochemical Basis of Signal versus Background; The Molecular-Based Assays; The Polymerase Chain Reaction; Southern Blot, Northern Blot, and Dot Blot Hybridization; Formalin Fixation and Molecular Hybridization; Immunohistochemistry
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Suggested Readings3 The Biochemical Basis of In Situ Hybridization and Immunohistochemistry; Introduction; The Biochemical Effects of Fixing Cells in Formalin; Cross-linking of Protein Side Chains to Other Proteins, DNA, RNA, and Glycoproteins; Pore Size and Biochemical Charges; Relationship of Biochemical Changes to Fixation Time and Temperature; Relationship of Biochemical Changes to Cell Type; The Biochemical Effects of Embedding the Tissue/Cells into Paraffin; DNA and RNA Nicks from the Paraffin Embedding Process; Alterations in Proteins
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The Biochemical Effects of Protease Digestion (Protease Pretreatment)Pore Size; Ionic Charge and Hydrogen Bond Potential of Cell/Breathability of the Macromolecules inside the Formalin-Fixed, Paraffin-Em ...; The Practical Significance of the Highly Variable "Formalin-Induced THREE-DIMENSIONAL Network" on Optimizing the Signal for ...; The Effect of Aging of the Tissue/Paraffin Block on the "Formalin-Induced THREE-DIMENSIONAL Network" for in Situ Hybridizat ...; Molecular Basis of the Loss of Signal with in Situ Hybridization and Immunohistochemistry Relative to the Aging of the Slid ...
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"Recharging" the Aged Tissue to Allow for Enhanced in Situ Hybridization and ImmunohistochemistrySummary; Suggested Readings; 4 The Basics of In Situ Hybridization; Introduction; Step One: Fixing the Tissue or Cells; Buffered Formalin-Fixed Cells and Tissue; The Use of Fixatives Other Than Formalin: Cross-Linking Fixatives; The Use of Fixatives Other than Formalin: Denaturing Fixatives; Adding Some Additional Ingredients to Formalin; Unfixed Cells/Tissue; A Quick Review of the Consequences of Formalin Fixation in Performing in Situ Hybridization
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Step Two: Putting the Fixed Cells or Tissue on a Glass Slide
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English
Weitere Ausg.:
ISBN 9780124159440
Weitere Ausg.:
ISBN 0124159443
Sprache:
Englisch
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