UID:
almafu_9958130300102883
Umfang:
1 online resource (207 p.)
ISBN:
0-323-16022-0
Inhalt:
Biological Applications of Liquid Scintillation Counting
Anmerkung:
Based on lectures given at the Worcester Foundation for Experimental Biology.
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Front Cover; Biological Applications of Liquid Scintillation Counting; Copyright Page; Table of Contents; Preface; CHAPTER 1. INTRODUCTION; Text; References; CHAPTER 2. PRINCIPLES OF LIQUID SCINTILLATION COUNTING; Scintillators and the Scintillation Process; Photomultiplier Tubes; The Single-Tube Liquid Scintillation Counter; The Two-Tube Liquid Scintillation Counter; The Beta Spectrum; Pulse Height Analysis; Balance Point Counting; Double-Isotope Analysis; Quenching; Efficiency Determination; Lesser Pulse Height Analysis; Figure of Merit; Logarithmic Logic Counters
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Automation and Data ProcessingReferences; CHAPTER 3. GENERAL CONSIDERATIONS OF SAMPLE PREPARATION; Scintillators; Secondary Scintillators; Solvents; Counting Solutions; Counting Vials; Sources of Background Counts; Counting in Solution; Emulsion Counting; Measurement of 14CO2; Combustion Techniques; Automated Combustion Apparatus; Spark Combustion; Suspension Counting; Counting on a Support Medium; Cerenkov Counting; References; CHAPTER 4. TESTING AND SETTING UP A NEW COUNTER; General Considerations; Testing Counters Based on Linear Pulse Height Analysis
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Testing Counters Based on Logarithmic Pulse Height AnalysisPlug-In Discriminators; The Chi-Square Test and Determination of Counter Stability; Determination of Counting Efficiency; Efficiency Determination in Heterogeneous Counting Systems; Curve Fitting; Counting Statistics; Automated Techniques for Sampling Counting Efficiency Determination; References; CHAPTER 5. BASIC PRINCIPLES OF THE APPLICATION OF RADIOTRACERS; Isotope Dilution; Derivative Analysis Using a Radioactive Reagent; Double-Isotope Derivative Analysis; Radiochemical Saturation Analysis
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Saturation Analysis Applied to Biological SystemsEvaluation of a Radioimmunoassay; Counting Gamma Emitters by Liquid Scintillation Counting; Enzyme-Isotope Dilution Analysis; Radioenzymatic Assays; References; CHAPTER 6. PRACTICAL ASPECTS OF SAMPLE PREPARATION; Tissues; Blood; Urine; Plasma; Water; Proteins; Amino Acids; Lipids; Carbohydrates; Steroids; Bacteria; Nucleic Acids; 14CO2 from a Warburg Flask; Nonautomatic Combustion Techniques; Polyacrylamide Gels; Miscellaneous; References; APPENDIX; Licensing Requirement; Some Useful Formulas
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How to Make up a Radioactive Solution to the Desired Specific ActivityDecontamination of Glass Counting Vials and Radioactive Glassware; Precision of Fixed-Time versus Fixed-Count Measurement; References; Index
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English
Weitere Ausg.:
ISBN 0-12-417250-4
Sprache:
Englisch
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