Format:
XI, 358 S. : Ill., graph. Darst.
ISBN:
0124262953
Content:
Contents: PART ONE: INTRODUCTION 1. Nucleic acid hybridization test formats: strategies and applications / Larry J. Kricka. 2. Nonradioactive labeling methods for nucleic acids / Christoph Kessler. PART TWO: DETECTION METHODS 3. Detection of alkaline phosphatase by time-resolved fluroescence / Eva Gudgin Templeton, Hector E. Wong, and Alfred Pollak. 4. Detection of alkaline phosphatase by bioluminescence / Reinhard Erich Geiger. 5. Detection of DNA on membranes with alkaline phosphatase-labeled probes and chemiluminescent AMPPD substrate / Annette.Tumulo, Owen J. Murphy, Quan Nguyen, John C. Voyta, Frank Witney, and Irena Bronstein. 6 Detection of alkaline phosphatase by colorimetry / Ayoub Rashtchian. 7. Detection of horseradish peroxidase by enhanced chemiluminescence / Ian Durrant. 8. Detection of horseradish peroxidase by colorimetry / Peter C. Verlander. 9. Detection of glucose 6-phosphate dehydrogenase by bioluminescence / Jean-Claude Nicolas, Patrick Balaguer, Béatrice Térouanne, Marie Agnès Villebrun, and Anne-Marie Boussioux. 10. Detection of lanthanide chelates by time-resolved fluorescence / Timo Lövgren, Pertti Hurskainen, and Patrik Dahlén. 11. Detection of lanthanide chelates and multiple labeling strategies based on time-resolved fluorescence / Eleftherios P. Diamandis and Theodore K. Christopoulos. 12. Detection of acridinium esters by chemiluminescence / Norman C. Nelson, Mark A. Reynolds, and Lyle J. Arnold, Jr. 13. Detection of energy transfer and fluorescence quenching / Larry E. Morrison.
Content:
Many nonisotopic methods of probing specific DNA sequences have been developed as replacements for radioactive labels, such as phosphorous and iodine. This book aims to bring these new methods together in one convenient, easily accessible source. It enables researchers to select the nonisotopic method best suited for their application and to maximize its advantage by following the instructions provided. The book contains chapters on colorimetric, bioluminescent, chemiluminescent, fluroescent and time-resolved fluorescent detection methods. Each chapter has been written by the inventor or developer of a particular nonisotopic method and thus provides an expert account of the method. Each chapter presents background information and detailed, easy-to-follow experimental procedures for labelling and detection.
Note:
MAB0014.001: AWI Bio-92-0244
,
2. Aufl. u.d.T.: Nonisotopic probing, blotting, and sequencing. - Includes bibliographical references and index
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