UID:
almafu_9960073270402883
Umfang:
1 online resource (441 p.)
ISBN:
1-281-02937-8
,
9786611029371
,
0-08-053577-1
Serie:
Biological techniques series
Inhalt:
Biological Techniques is a series of volumes aimed at introducing to a wide audience the latest advances in methodology. The pitfalls and problems of new techniques are given due consideration, as are those small but vital details not always explicit in the methods sections of journal papers.In recent years, most biological laboratories have been invaded by computers and a wealth of new DNA technology and this will be reflected in many of the titles appearing in the series.The books will be of value to advances researches and graduate students seeking to learn and apply new tech
Anmerkung:
Description based upon print version of record.
,
Front Cover; Manual of Techniques in Insect Pathology; Copyright Page; Contents; Series Preface; Contributors; Preface; Acknowledgements; Chapter 1. Initial handling and diagnosis of diseased insects; 1 Introduction; 2 Collection of diseased insects and entomopathogens; 3 Examination in the laboratory; 4 Safety considerations; Acknowledgements; References; Glossary; Chapter II. Viruses; 1 Introduction; 2 A brief introduction to the insect virus groups; 3 Identification; 4 Isolation; 5 Quantification of viruses; 6 Propagation; 7 Bioassay; 8 Preservation and long-term storage; Acknowledgements
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ReferencesGlossary; Appendix; Chapter III-1. Bacteria: Identification, isolation, culture and preservation of entomopathogenic bacteria; 1 Introduction; 2 Identification; 3 Isolation; 4 Cultivation; 5 Preservation; References; Appendix; Chapter III-2. Bacteria: Laboratory bioassay of bacteria against aquatic insects with emphasis on larvae of mosquitoes and black flies; 1 Introduction; 2 Bioassay of Bacillus pathogens against mosquito larvae; 3 Bioassay of Bacillus thuringiensis isolates against black fly larvae; 4 Conclusions; Acknowledgements; References
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Chapter III-3. Bacteria: Bioassay of Bacillus thuringiensis against lepidopteran larvae1 Introduction; 2 Diet-based bioassays; 3 Bioassay of granular formulations of B. thuringiensis; 4 Bioassay of sprayable formulations of B. thuringiensis on leaf surfaces; 5 Conclusions; References; Chapter III-4. Bacteria of soil-inhabiting insects; 1 Introduction; 2 Isolation; 3 Identification; 4 Propagation; 5 Bioassay; 6 Preservation; References; Appendix; Chapter IV. Research methods for entomopathogenic protozoa; 1 Introduction; 2 Identification; 3 Bioassay and experimental infections
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4 Propagation and production5 Managing spores; 6 Storage; References; Chapter V-1. Fungi: Identification; 1 Introduction; 2 Preparation and observation of microscope slides; 3 Key to major genera of fungal entomopathogens; 4 Diagnoses and critical characters of major entomopathogens; References; Brief glossary of mycological terms; Appendix: recipes of stains and reagents; Chapter V-2. Fungi: Entomophthorales; 1 Introduction; 2 Isolation; 3 Culture and production; 4 Bioassays; 5 Sampling Entomophthorales; Acknowledgements; References; Appendix: Media; Chapter V-3. Fungi: Hyphomycetes
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1 Introduction2 Isolation and identification; 3 Propagation and storage; 4 Propagule enumeration; 5 Infection and bioassay; 6 Assessment of environmental parameters; Acknowledgements; References; Appendix; Chapter V-4. Fungi: Oomyeetes and Chytridiomycetes; 1 Introduction: phylogeny and life cycles; 2 Isolation; 3 Propagation; 4 Bioassay; 5 Conclusions; Acknowledgements; References; Appendix; Chapter V-5. Fungi: Preservation of cultures; 1 Introduction; 2 Preservation at temperatures above freezing; 3 Preservation by freeze-drying (lyophilization); 4 Preservation in a frozen state; References
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Chapter VI. Techniques in insect nematology
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English
Weitere Ausg.:
ISBN 0-12-432555-6
Sprache:
Englisch
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