UID:
edoccha_9958128648402883
Format:
1 online resource (424 p.)
ISBN:
1-281-75556-7
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9786611755560
,
0-08-085956-9
Series Statement:
Methods in cell biology, v. 58
Content:
This volume is an authoritative and comprehensive treatment of the approaches and techniques used for Green Fluorescent Proteins (GFP). The primary focus of this work is on research using biological systems. The volume covers all aspects of GFP, from its expression in different organisms to specific microscopic and data analysis methods.Key Features* Only volume on Green Fluorescent Protein research* Covers all aspects of GFP* Provides specific microscopic and data analysis methods* Discusses the design and construction of GFP fusion proteins* Covers GFP expression
Note:
"Prepared under the auspices of the American Society for Cell Biology."
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Front Cover; Methods in Cell Biology; Copyright Page; Contents; Contributors; Preface; Chapter I. Biophysics of the Green Fluorescent Protein; I. Introduction; II. Protein Folding and the Generation of This Chromophore; III. The Biophysics of the Fluorescence of GTP; IV. Resonance Energy Transfer Involving GFP; V. Summary; References; Chapter 2. Understanding Structure-Function Relationships in the Aequorea Victoria Green Fluorescent Protein; I. Introduction; II. Structure; III. Chromophore Formation; IV. Effects of Mutations on the Spectroscopic Properties of GFP
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V. Effects of Mutations That Improve ThermosensitivityVI. The Development of Enhanced Mutants; References; Chapter 3. Quantitative Imaging of the Green Fluorescent Protein (GFP); I. Introduction; II. Factors That Influence/Limit Quantitation of GFP in Fluorescence Microscopy; III. Applications of LSCM for Quantitative Imaging of GFP; IV. Preparation of Purified GFP Samples; References; Chapter 4. Single-Molecule Fluorescence Detection of Green Fluorescence Protein and Application to Single-Protein Dynamics; I. Introduction
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II. Design Considerations for Fluorescence Microscopes for Single-Molecule DetectionIII. Characteristics of the Fluorescence from Single GFP Molecules; IV. Advantages of Using GFP for Single-Molecule Detection; V. GFP in Vitro and in Vivo Assays; Appendix I. Details of the TIR Microscope; Appendix II. Data Acquisition and Analysis; References; Chapter 5. Targeting GFP to Organelles; I. Introduction; II. Construction and Expression of the Organelle-Targeted GFP Chimeras; III. Dynamic Monitoring of Organelle Structure with the Targeted GFPs; IV. Expression in Primary Cultures
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V. Visualizing GFP Chimeras with Different Spectral PropertiesVI. Protocols; References; Chapter 6. Cytoskeletal Dynamics in Yeast; I. Introduction; II. Generating GFP Fusions; III. Imaging Considerations for Yeast Cells; IV. Time-Lapse Microscopy; V. Results: Cytoskeletal GFP Fusion Proteins; VI. Future; References; Chapter 7. Analysis of Nuclear Transport in Vivo; I. Introduction; II. Experimental Approaches and Protocols; References; Chapter 8. GFP Fusion Proteins as Probes for Cytology in Fission Yeast; I. Introduction; II. Expressing GFP Fusion Proteins
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III. Applications of Fusion ProteinsReferences; Chapter 9. GFP Variants for Multispectral Imaging of Living Cells; 1. Introduction; II. Green Fluorescent Protein Markers; III. Imaging of Living Cells; IV. Marking Different Cell Types in Arabidopsis; V. Spectrally Distinct Fluorescent Proteins for Multichannel Confocal Microscopy; VI. Summary; References; Chapter 10. GFP Fusions to a Microtubule Motor Protein to Visualize Meiotic and Mitotic Spindle Dynamics in Drosophila; I. Introduction; II. Labeling Strategies; III. Imaging GFP; IV. Applications of Ncd-GFP Imaging; V. Perspectives
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References
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English
Additional Edition:
ISBN 0-12-544160-6
Language:
English
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