UID:
edoccha_9958132582102883
Umfang:
1 online resource (435 p.)
ISBN:
1-281-51482-9
,
9786611514822
,
0-08-085957-7
Serie:
Methods in cell biology ; v. 59
Inhalt:
This is the first in a two-volume, comprehensive treatment of the methodologies used in researching the Zebrafish, an emerging vertebrate model system. The text includes discussions on development, genetic methodologies, and model applications.Key Features* Details state-of-the-art zebrafish protocols in a single-source reference* Presents methods and reagents in user-friendly format* Delineates critical steps and pitfalls of he procedures* Illustrates techniques with full-color plates* Summarizes many new and interesting developmental mutants* Includes appendic
Anmerkung:
"Prepared under the auspices of the American Society for Cell Biology."
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Front Cover; Methods in Cell Biology, Volume 59; Copyright Page; Contents; Contributors; Preface; PART I: Introduction; Chapter 1. Overview of the Zebrafish System; I. Introduction; II. History of the Zebrafish System and Its Advantages and Disadvantages; III. Cell and Developmental Biology, Organogenesis, and Human Disease; IV. Genetics and Genomics; V. Future Prospects; VI. Conclusion; References; Chapter 2. Cell Cycles and Development in the Embryonic Zebrafish; I. Introduction; II. Terminology and the Staging Series; III. The Zygote Period; IV. The Cleavage Period; V. The Blastula Period
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VI. The Gastrula PeriodVII. The Segmentation Period; References; PART II: Cell Culture and General Methods; Chapter 3. Zebrafish Embryonal Cell Culture; I. Introduction; II. General Cell Culture Considerations; III. Primary and Multipassage Zebrafish Embryo Cell Culture; IV. Transfection; V. Applications to Other Fish Models; VI. Conclusion; References; Chapter 4. Primary Fibroblast Cell Culture; I. Introduction; II. Material and Methods; III. Results and Discussion; References
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Chapter 5. Production of Haploid and Diploid Androgenetic Zebrafish (Including Methodology for Delayed in Vitro Fertilization)I. Introduction; II. Equipment and Materials; III. Methods; IV. Results and Discussion; V. Conclusions and Perspectives; References; PART III. Gene Expression and Function in Development; Chapter 6 . Analysis of Protein and Gene Expression; I. In Situ Hybridlzation to RNA and Immunolocalization of Proteins; II. Probe Synthesis; III. Fixation; IV. Hybridization to Whole-Mount Embryos; V. Immunolocalization of Probes; VI. Two-Color in Sit# Hybridization
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VII. Double-Fluorescent in Situ HybridizationVIII. Simultaneous Localization of Transcription and Translation Gene Products; IX. Embedding and Sectioning Whole-Mount Embryos; X. Solutions and Reagents; References; Chapter 7. Strategies to Perturb Zebrafish Development; I. Introduction; II. Administration of Reagents; III. Reagents; IV. Experiments to Modify the Activity of Specific Gene Products; V. Perspectives: Inducible Systems; References; Chapter 8. Vectors and Techniques for Ectopic Gene Expression in Zebrafish; I. Introduction; II. Misexpression from DNA; III. Misexpression from RNA
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ReferencesChapter 9. Analysis of Zebrafish Development Using Explant Culture Assays; I. Introduction; II. Zebrafish Explants: General Considerations; III. Materials Required; IV. Guide to Explant Isolation and Culture; V. Using Explants to Assay Induction; VI. Illustrations of Specification and Induction Assays; VII. Future Directions; References; PART IV: Early Embryonic Development; Chapter 10. Embryonic Axis Formation in the Zebrafish; I. The First Signs of Dorsal; II. Establishment of Dorsal-Ventral Polarity in Blastula Stages; III. Dorsal-Ventral Patterning
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IV. Development of Midline Mesodermal Tissues
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English
Weitere Ausg.:
ISBN 0-12-544161-4
Sprache:
Englisch
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