UID:
almafu_9958129965802883
Umfang:
1 online resource (478 p.)
ISBN:
1-281-02338-8
,
9786611023386
,
0-12-044142-X
,
0-08-052496-6
Serie:
Methods in cell biology, v. 69
Inhalt:
Critically acclaimed for more than 25 years, the Methods in Cell Biology series provides an indispensable tool for the researcher. Each volume is carefully edited by experts to contain state-of-the-art reviews and step-by-step protocols. Techniques are described completely so that methods are made accessible to users. This volume, Methods of Cell-Matrix Adhesion, contains integrated coverage on cell-matrix adhesion methods. It brings the classical methodologies and the latest techniques together in one concise volume. This coverage includes experimental protocols and their conce
Anmerkung:
Description based upon print version of record.
,
Cover; Copyright Page; Contents; Contributors; Acknowledgments; Part I: Preface and Perspectives on Cell-Matrix Adhesion; Preface; Cell-Matrix Interactions: The View from the Outside; Matrix and Meaning; Cell-Matrix Adhesion Research and the Development of Biotherapeutics; Part II: Matrix Methodologies; Chapter 1. Detection and Purification of Instructive Extracellular Matrix Components with Monoclonal Antibody Technologies; I. Introduction; II. Methods for Detecting ECM Components That Instruct Cell Responses in Reporter Cells; III. Preparation of Mabs for Identification of Instructive ECM
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IV. Purification of Instructive ECM with Mabs and Interactions with Reporter CellsV. Summary; References; Chapter 2. Isolation and Purification of Proteoglycans; I. Overview and Scope; II. Extraction of Proteoglycans; III. Radiolabeling of Proteoglycans for Ease of Purification; IV. Anion-Exchange Chromatography; V. Hydrophobic Interaction Chromatography; VI. Gel Filtration Chromatography; VII. Immunoaffinity Chromatography; VIII. Quantification and Characterization; References; Chapter 3. Expression of Recombinant Matrix Components Using Baculoviruses
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I. Introduction: The Importance of Recombinant Extracellular Matrix Molecules for Protein StudiesII. Requirements and Rationale for Development of a General Protein Expression System; III. Expression in Insect Cells Using pCOCO; IV. Results Obtained from the COCO System; References; Chapter 4. Heparan Sulfate-Growth Factor Interactions; I. Introduction: HS Regulation of Growth Factor Signaling; II. Methods for Identifying the Role of HS in Growth Factor Binding and Signaling; III. Identification of Regulatory HS; IV. Analyzing the Structure of HS: General Considerations and Methodologies
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ReferencesChapter 5. Analysis of Basement Membrane Self-Assembly and Cellular Interactions with Native and Recombinant Glycoproteins; I. Introduction; II. Native and Recombinant Basement Membrane Proteins; III. Analysis of Self-Assembly in Vitro; IV. Analysis of Cell-Surface Contributions to Basement Membrane Assembly; V. Approaches to Evaluating ECM Molecules for Assembly Interactions; VI. Integration of Genetic and Molecular Approaches; References; Chapter 6. Preparation and Analysis of Synthetic Multicomponent Extracellular Matrix; I. Introduction to the ECM
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II. Development of Synthetic MatricesIII. Discussion; References; Chapter 7. Analysis of Matrix Dynamics by Atomic Force Microscopy; I. Introduction; II. General Experimental Design; III. Probe Microscopy in Fluid: AFM Imaging; IV. Probe Microscopy in Fluid: Molecular Pulling/Force Spectroscopy; V. Probe Microscopy in Air: AFM Imaging; VI. Conclusion; References; Chapter 8. Analysis of Matrix Degradation; I. Introduction; II. Experimental Approaches and Protocols; References; Part III: Adhesion Receptor Methodologies
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Chapter 9. Expression Cloning Strategies for the Identification of Adhesion Molecules
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English
Weitere Ausg.:
ISBN 0-12-544168-1
Sprache:
Englisch
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