UID:
almafu_9958127467702883
Format:
1 online resource (304 p.)
ISBN:
0-323-14061-0
,
1-299-19466-4
Content:
This guide for the practicing chromatographer who wants a ready source of information on HPLC detection explores and compares existing detection systems and detectors, outlines the common problems associated with a given detector, and offers proven approaches to avoiding such problems.Key Features* Addresses the practical aspects of HPLC detection, including:basic theory* when a particular type of detector can be used* how detectors from various manufacturers differ* common problems of detectors and ways to avoid them* Presents an overview of today's most
Note:
Description based upon print version of record.
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Front Cover; A Practical Guide to HPLC Detection; Copyright Page; Table of Contents; CHAPTER 1. INTRODUCTION; References; CHAPTER 2. REFRACTIVE INDEX DETECTION; I. Introduction; II. Two Types of RI Detectors; III. The Physical Property; IV. Refractive Index of Binary Mixtures; V. Minimum Detectability; VI. Linear Dynamic Range; VII. Problems with Changing Solvent Composition; Acknowledgment; References; CHAPTER 3. ABSORBANCE DETECTION; I. Overview of Absorbance Detectors; II. Instrumentation; III. Quantitative Measurement; IV. Troubleshooting; References
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CHAPTER 4. PHOTODIODE ARRAY ABSORBANCE DETECTIONI. Introduction; II. Theory; III. Optics; IV. Data Output; V. Applications of Diode Array Detectors; VI. Summary; Bibliography/References; CHAPTER 5. FLUORESCENCE DETECTION; I. Introduction; II. Photophysical Processes of Molecules in Solution; III. Chemical Effects on Fluorescence; IV. Fluorescence Detector Instrument Designs; V. Signal-to-Noise Ratio Considerations; VI. Quantitation; VII. Fluorometric Derivatization in HPLC; VIII. Chemiluminescence; References; CHAPTER 6. ELECTROCHEMICAL DETECTION; I. Introduction; II. Amperometric Detection
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III. Conductivity DetectionIV. Conclusion; References; CHAPTER 7. MASS SPECTROMETRY AS AN LC DETECTION TECHNIQUE; I. Introduction; II. MS as an LC Detector; III. LC/MS Interfacing Techniques; IV. Summary and Selected Applications; References; CHAPTER 8. POST-COLUMN DERIVATIZATION TECHNIQUES; I. Introduction; II. Scope of Post-Column Reaction Detection; III. Characteristics of Post-Column Reactions; IV. Post-Column Reactor System Design; V. Reactions Suitable for Post-Column Reaction Detection; VI. Conclusion; References; CHAPTER 9. OTHER MODES OF DETECTION; I. Introduction; II. Radioactivity
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III. Infrared DetectionIV. Light-Scattering Detection; V. Optical Activity; VI. Other Detectors; References; INDEX
,
English
Additional Edition:
ISBN 0-12-545680-8
Language:
English
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