UID:
almafu_9958126732902883
Format:
1 online resource (391 p.)
ISBN:
9786611514174
,
9781281514172
,
1281514179
,
9780080859491
,
0080859496
Series Statement:
Methods in cell biology, v. 51
Content:
A current and authoritative guide, Methods in Avian Embryology presents a combination of classical embryological techniques and modern molecular biological approaches to studying the developing avian embryo. The only one of its kind, this book is specifically devoted to providing a detailed approach to studying avian embryos. It also describes how to use this system to study problems in cell, developmental, and neurobiology. The protocols emphasize microsurgery, histology, and cellular and molecular marking, which are not covered in the usual molecular biology methods manuals. The metho
Note:
Description based upon print version of record.
,
Front Cover; Methods in Cell Biology, Volume 51; Copyright Page; Contents; Contributors; Preface; Chapter 1. Culture and Microsurgical Manipulation of the Early Avian Embryo; I. General Introduction; II. Culture of the Avian Embryo; III. Tools for Microsurgery; IV. Microsurgical Approaches to the Young Embryo; References; Chapter 2. Quail-Chick Transplantations; I. Introduction; II. Differential Diagnosis of Quail and Chick Cells; III. Material and Equipment; IV. Preparation and Sealing of Eggs; V. Neural Tissue Transplantations; VI. Early Transplantations in Blastodiscs
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VII. Hemopoietic Organ Rudiment TransplantationsVIII. Results, Discussion, and Perspectives; References; Chapter 3. Manipulations of Neural Crest Cells or Their Migratory Pathways; I. Introduction; II. Preparation of Avian Neural Crest Cultures; III. Microinjection of Cells and Antibodies into Embryos; IV. Labeling of Neural Crest Cells in Vivo with Vital Dyes; V. Grafting Techniques; VI. Conclusions; References; Chapter 4. Manipulation of the Avian Segmental Plate in Vivo; I. Introduction; II. Materials; III. Methods; IV. Critical Aspects of the Procedure; V. Results and Discussion
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VI. Conclusions and PerspectivesReferences; Chapter 5. Somite Strips: An Embryo Fillet Preparation; I. Introduction; II. Fillet Preparation; III. Critical Aspects; IV. Analyzing Neurites to Assess Guidance Interactions; V. Conclusions and Perspectives; References; Chapter 6. Embryo Slices; I. Introduction; II. Cutting and Culturing Embryo Slices; III. Embryo Slice Characteristics; IV. Experimental Results Using Embryo Slices; V. Conclusions and Perspectives; References; Chapter 7. Operations on Limb Buds of Avian Embryos; I. Introduction; II. Preparing to Operate
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III. Extraembryonic MembranesIV. Making Various Kinds of Grafts; V. Manipulations Involving the Apical Ectodermal Ridge; VI. Grafts to Test Polarizing Activity; VII. Enzymatic Dissociation and Recombination of Limb Tissues; References; Chapter 8. Iontophoretic Dye Labeling of Embryonic Cells; I. Introduction; II. Iontophoretic Microinjection of Lineage Tracers; III. Iontophoretic Application of DiI; IV. Relative Advantages of Dextran and DiI; References; Chapter 9. Gene Transfer Using Replication-Defective Retroviral and Adenoviral Vectors; I. Introduction; II. Retroviral Vectors
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III. Adenoviral VectorsIV. Injection of Virus into Chick Embryos; V. Histology and Histochemistry; VI. Uses of Viral Vectors; References; Chapter 10. Manipulating Gene Expression with Replication-Competent Retroviruses; I. Introduction; II. Materials; III. Methods; IV. Results and Discussion; V. Conclusions and Perspectives; References; Chapter 11. In Situ Hybridization Analysis of Chick Embryos in Whole Mount and Tissue Sections; I. Introduction; II. When to Hybridize to Sections or Whole Mounts; III. Solutions; IV. Whole Mount in Situ Hybridization; V. Double Detection of Two RNAs
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VI. Double Detection of RNA and Protein
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English
Additional Edition:
ISBN 9780125641531
Additional Edition:
ISBN 0125641532
Language:
English
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