UID:
edoccha_9958127989002883
Umfang:
1 online resource (521 p.)
Ausgabe:
2nd ed.
ISBN:
0-08-092535-9
,
1-281-01209-2
,
9786611012090
,
0-12-580445-8
,
0-08-049658-X
Serie:
Methods in cell biology ; 70
Inhalt:
Confocal Microscopy contains integrated and up-to-date coverage on the many various techniques and uses of the confocal microscope (CM). Techniques are described completely so that methods are made accessible to users. This is a must-have reference for all CM users.Key Features* Includes detailed protocols accessible to new users * Details how to set up and run a ""Confocal Microscope Core Facility""* Contains over 170 figures
Anmerkung:
Description based upon print version of record.
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Front Cover; Methods in Cell Biology; Copyright Page; Contents; Contributors; Preface; Chapter 1. Introduction to Confocal Microscopy; I. Introduction; II. Principle of Confocal Microscopy; III. Major Components of a Confocal microscope; IV. Designs of Confocal Microscopes; V. Choosing and Setting Up a Confocal Microscope System; VI. Specimen Preparation; VII. Applications of Confocal Microscopy; VIII. Alternatives to Confocal Microscopy; IX. Conclusions and Future Directions; X. Resources for Confocal Microscopy; References; Chapter 2. Direct-View High-Speed Confocal Scanner: The CSU-10
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I. IntroductionII. Overview of Confocal Microscopes; III. Design of the CSU-10; IV. Sample Biological Applications; V. Low Bleaching of Fluorescence Observed with the CSU-10; VI. Very-High-Speed Full-Frame Confocal Imaging with the CSU-10; VII. Haze Removal, Image Sharpening, and Dynamic Stereo Image Generation by Digital Signal Processing; VIII. Mechanical and Optical Performance of the CSU-10; IX. Potentials of the CSU-10; X. Addendum A (April, 2002); XI. Addendum B (personal communication from Dr. Kenneth R. Spring, June, 2002); References
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Chapter 3. Introduction to Multiphoton Excitation Imaging for the Biological SciencesI. Introduction; II. Principles of Multiphoton Imaging; III. Advantages of Multiphoton Imaging; IV. Imaging Parameters; V. Applications of Multiphoton Imaging; VI. New Development in Nonlinear Optics; VII. Conclusion; References; Chapter 4. Confocal Microscopy: Important Considerations for Accurate Imaging; I. Introduction; II. Factors Affecting Confocal Imaging; III. Conclusions; References; Chapter 5. Multicolor Laser Scanning Confocal Immunofluorescence Microscopy: Practical Application and Limitations
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I. IntroductionII. Immunofluorescence Histochemistry; III. Instrumentation; IV. Approaches to Multicolor Laser Scanning Confocal Microscopy; V. Practical Aspects of Multicolor Laser Scanning Confocal Microscopy; VI. Multicolor Laser Scanning Confocal Microscopy Considerations; VII. Conclusions; References; Chapter 6. Practical Aspects of Objective Lens Selection for Confocal and Multiphoton Digital Imaging Techniques; I. Introduction; II. Confocal Microscopy Resolution; III. Infinity versus Finite Lens Systems; IV. Objective Lens Nomenclature and Features; V. Objective Lens Aberrations
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VI. Dry (Nonimmersion) and Immersion Objective LensesVII. Epi (Reflected Light) Objectives; VIII. Live Cell and Fixed Tissue Confocal Applications and Objective Lens Selection; IX. Possible Solutions for Potential Problematic Applications; X. Optimum Objective Lenses for Multiphoton Applications; XI. Transmission Data for Confocal and Multiphoton Objectives; XII. Conclusions; References; Chapter 7. Resolution of Subcellular Detail in Thick Tissue Sections: Immunohistochemical Preparation and Fluorescence Confocal Microscopy
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I. Introduction: Development of Fluorescence Microscopy Techniques for Resolution of Subcellular Detail
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English
Weitere Ausg.:
ISBN 0-12-480277-X
Sprache:
Englisch
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