Format:
Online Ressource (12250 KB, XV, 327 S.)
,
Ill., graph. Darst.
Edition:
1. Aufl.
Edition:
Online-Ausg.
ISBN:
0128015128
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1336029420
,
9780128015124
,
9781336029422
Series Statement:
Methods in enzymology 554
Content:
These new volumes of Methods in Enzymology (554 and 555) on Hydrogen Sulfide Signaling continue the legacy established by previous volumes on another gasotransmitter, nitric oxide (Methods in Enzymologyvolumes 359, 396, 440, and 441), with quality chapters authored by leaders in the field of hydrogen sulfide research. These volumes of Methods in Enzymologywere designed as a compendium for hydrogen sulfide detection methods, the pharmacological activity of hydrogen sulfide donors, the redox biochemistry of hydrogen sulfide and its metabolism in mammalian tissues, the mechanisms inherent in hydrogen sulfide cell signaling and transcriptional pathways, and cell signaling in specific systems, such as cardiovascular and nervous system as well as its function in inflammatory responses. Two chapters are also devoted to hydrogen sulfide in plants and a newcomer, molecular hydrogen, its function as a novel antioxidant. Continues the legacy of this premier serial with quality chapters on hydrogen sulfide research authored by leaders in the field Covers conventional and new hydrogen sulfide detection methodsCovers the pharmacological activity of hydrogen sulfide donorsContains chapters on important topics on hydrogen sulfide modulation of cell signaling and transcriptional pathways, and the the role of hydrogen sulfide in the cardiovascular and nervous systems and in inflammation ...
Note:
Description based upon print version of record
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Front Cover; Hydrogen Sulfide in Redox Biology, Part A; Copyright; Contents; Contributors; Preface; Section I: Hydrogen Sulfide Detection Methods; Chapter 1: Mechanistic Chemical Perspective of Hydrogen Sulfide Signaling; 1. Introduction; 2. Bioavailability of Sulfide-The Signal; 2.1. Endogenous sulfide production; 2.2. Sulfide catabolism; 2.3. Endogenous sulfide buffers; 3. Inorganic Polysulfides; 3.1. Biological relevance; 3.2. Speciation and redox capacity of polysulfides; 3.3. Polysulfide formation by sulfide oxidation; 3.4. Stability of polysulfides
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4. Sulfide Signaling Via Protein Sulfhydration4.1. Mechanisms of persulfide formation; 4.1.1. Persulfide formation via disulfide reduction; 4.1.2. Persulfide formation via the reactions of Cys sulfenic acid species with sulfide; 4.1.3. Persulfide formation via the reactions of oxidized sulfide species with Cys thiols; 4.1.4. Persulfide formation via radical pathways; 5. Sulfide Signaling via Sulfide-Hemeprotein Interactions; 5.1. Sulfide mediates heme protein functions; 5.2. Heme proteins generate sulfide oxidation products
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5.3. Antioxidant properties of sulfide via reduction of metal centers with higher oxidation states6. Conclusions; Acknowledgments; References; Chapter 2: Measurement of H2S In Vivo and In Vitro by the Monobromobimane Method; 1. Introduction; 1.1. Properties of hydrogen sulfide; 1.2. Hydrogen sulfide pools; 1.3. Physiological and pathophysiological roles of hydrogen sulfide; 1.4. Measurement of hydrogen sulfide bioavailability; 2. Experimental Methods; 2.1. Derivatization reaction of H2S with monobromobimane; 2.1.1. Procedure; 2.1.2. Comment and limitations
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2.2. H2S detection in biological samples: Effects of sample preparation2.3. RP-HPLC with fluorescence detection; 2.3.1. Procedure; 2.3.2. Preparation of SDB standard; 2.3.3. Comment and limitations; 2.4. H2S and sulfide pool detection in biological samples; 2.4.1. Procedure; 2.4.2. Comment and limitations; 2.5. Confirmation of HPLC and SDB by mass spectrometer; 2.5.1. Procedure; 2.5.2. Comment and limitations; 3. Summary; Acknowledgment; References; Chapter 3: Hydrogen Sulfide Detection Using Nucleophilic Substitution-Cyclization-Based Fluorescent Probes; 1. Introduction
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2. Design and Synthesis of the Probes3. Chemistry and Properties of the Probes; 3.1. Materials; 3.2. Test the reaction between the probes and H2S; 3.3. Fluorescence turn-on properties by H2S; 3.4. Test the probes selectivity for H2S; 4. Applications of the Probes in H2S Imaging in Cell-Based Experiments; 4.1. Materials; 4.2. Fluorescence imaging of exogenous H2S in HeLa cells; 4.3. Fluorescence imaging of H2S generated by persulfide-based H2S donors; 4.4. Fluorescence imaging of H2S generated from photo-sensitive H2S donors; 5. Conclusions; Acknowledgments; References
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Chapter 4: Azide-Based Fluorescent Probes: Imaging Hydrogen Sulfide in Living Systems
Additional Edition:
ISBN 012801623X
Additional Edition:
ISBN 9780128016237
Additional Edition:
Print version Hydrogen Sulfide in Redox Biology Part A
Language:
English
Keywords:
Schwefelwasserstoff
;
Redoxreaktion
;
Aufsatzsammlung
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