Format:
1 Online-Ressource (xx, 445 Seiten)
,
Illustrationen, Diagramme
Edition:
First edition
ISBN:
9780128053829
,
0128054352
,
9780128054352
Series Statement:
Methods in enzymology volume 579
Content:
Front Cover -- The Resolution Revolution: Recent Advances In cryoEM -- Copyright -- Contents -- Contributors -- Preface -- References -- Chapter One: Direct Electron Detectors -- 1. Introduction -- 2. Past -- 3. Present -- 3.1. Practical Advice for the User -- 4. Future -- References -- Chapter Two: Specimen Behavior in the Electron Beam -- 1. Introduction -- 2. High-Energy Electrons Are a Form of Ionizing Radiation as Well as Being a Form of Short-Wavelength Radiation That Can B ... -- 2.1. Electron-Scattering Events Can Be Either Elastic or Inelastic
Content:
2.2. Energy Is Deposited in the Specimen as a Result of Inelastic Scattering -- 2.3. Values of the Linear Energy Transfer (LET) Can Be Used to Estimate the Energy Deposited -- 3. Biological Molecules Become Structurally Damaged When Irradiated -- 3.1. There Is a Large Literature of Radiation Chemistry and Radiation Biology -- 3.2. Fading of Diffraction Patterns Is a Convenient Indicator of Structural Damage -- 3.3. Some Residues in Proteins Are Especially Sensitive to Radiation Damage
Content:
3.4. Caging of Fragments and ``Trapping´´ of Radicals Results in Cryo-Protection: This Helps Only to a Limited Extent -- 3.5. Radiation Sensitivity of Enzyme Activity: Implications for Dynamic Studies in Liquid Samples -- 4. Vitreous Ice Also Becomes Structurally Damaged by Ionizing Radiation -- 4.1. Water Molecules Are Easily Damaged by Ionizing Radiation -- 4.2. Weak Thon Rings at High Resolution Show That Vitreous Ice Is Very Sensitive to Radiation Damage -- 4.3. Electron-Stimulated Desorption Progressively Thins Ice Specimens
Content:
5. Bubbling of Hydrated Biological Specimens Becomes Apparent at High Electron Exposure -- 5.1. Bubbles Consist of Molecular Hydrogen -- 5.2. Bubbling Can Be Used to Evaluate the Specimen Thickness -- 5.3. Bubbling Can Be Used to Distinguish Regions with Different Chemical Composition (Bubblegrams) -- 6. Cryo-Specimens Exhibit Collective Beam-Induced Movement When Irradiated -- 6.1. Radiation-Sensitive Specimens Show Beam-Induced Motion at Quite Low Electron Exposures -- 6.2. Thin Cryo-Specimens Undergo Drum-Head-Like Flexing and Doming When Irradiated
Content:
6.3. Images Can Be Corrupted Significantly by There Being Changes in Z-Height -- 6.4. The Pattern of Beam-Induced Movement Can Be Quite Unpredictable -- 7. More Than One Mechanism May Contribute to Beam-Induced Motion -- 7.1. Cryo-EM Specimens, as Made, Are Expected to Be Under Considerable Stress -- 7.2. Irradiation Can Relieve Mechanical Stress -- 7.3. Irradiation Can Generate (New) Mechanical Stress -- 7.4. Which Comes First, Relaxation or Creation of Stress? -- 8. Irradiation Can Produce Electrostatic Charging of the Specimen
Content:
8.1. A Buildup of Net Charge on the Specimen Can Be Easy to Detect
Note:
Description based upon print version of record
Additional Edition:
Print version Crowther, R. A The Resolution Revolution: Recent Advances In cryoEM Saint Louis : Elsevier Science,c2016 ISBN 9780128053829
Language:
English
Subjects:
Biology
Keywords:
Kryoelektronenmikroskopie
;
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