UID:
almahu_9949983017602882
Umfang:
1 online resource (272 pages)
ISBN:
9780128189993
,
0128189991
Anmerkung:
Front cover -- Half title -- Full title -- Copyright -- Contents -- Preface -- Chapter 1 - Fundamentals of imaging mass spectrometry -- Principles of imaging mass spectrometry -- Advantages and limitation of imaging mass spectrometry -- References -- Chapter 2 - Ionization sources for imaging mass spectrometry -- Matrix-assisted laser desorption/ionization -- Desorption electrospray ionization -- MALDI-2 -- Laser ablation electrospray ionization -- Secondary ion MS -- Laser ablation inductively coupled plasma -- Liquid extraction surface analysis -- Nanospray desorption electrospray ionization -- Conclusions -- References -- Chapter 3 - Sample preparation for imaging mass spectrometry -- Sectioning and storage -- Mounting -- Imprinting and stamping -- Tissue storage -- Tissue drying -- Tissue rinsing and incubation -- Tissue washing -- Deparaffinization -- Pre-extraction -- Other tissue incubation procedures -- On-tissue chemistry -- On-tissue chemical derivatization -- Antigen retrieval -- Immunohistochemistry -- On-tissue in situ enzymatic digestion -- Heat stabilization of tissue -- In-plume microdroplet reactions -- Adding chemicals to tissue -- Chemical doping -- Matrix for MALDI -- Conclusions -- References -- Chapter 4 - Tissue sectioning for imaging mass spectrometry -- Tissue collection -- Snap freezing -- Fixing or embedding -- Tissue orientation -- Cryosectioning -- Mounting -- Tissue section storage -- Conclusions -- References -- Chapter 5 - Matrix for matrix-assisted laser desorption/ionization (MALDI) -- Matrix application -- Manual matrix application by airbrushing -- Automated matrix deposition -- Application of matrix by sublimation -- Precoated matrix slides -- Dry-coating matrix -- Types of matrices for MALDI -- Nanomaterial as a matrix -- Reactive matrix -- Matrix selection -- Matrix stability -- Conclusions.
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References -- Chapter 6 - Molecule identification approaches in imaging mass spectrometry -- Accurate mass matching -- Orthogonal identifier-collision cross section -- Fragmentation by ion dissociations -- Secondary MS analysis -- Immunolabeling -- Conclusions -- References -- Chapter 7 - Strategies for quantitative imaging mass spectrometry -- Normalization -- Global normalization -- Internal standard normalization -- Tissue-specific normalization coefficient -- Quantitation level in imaging MS -- Relative quantitation -- Absolute quantification -- Absolute quantification calibration strategies -- Off-tissue calibration -- On-tissue calibration -- Mimetic tissue calibration -- Offline and other tissue quantitation strategy -- Conclusions -- References -- Chapter 8 - Spatial resolution of imaging mass spectrometry -- Spatial resolution in biomedical imaging -- Numbers of pixels for spatial resolution -- Spatial resolution in laser desorption imaging MS -- Measuring spatial resolution in imaging MS -- Analyte-dependent pixel size or spatial resolution -- Conclusions -- References -- Chapter 9 - Visualization in imaging mass spectrometry -- Colormaps -- Colormap scale -- Image interpolation -- Conclusions -- References -- Chapter 10 - Data analysis and computation for imaging mass spectrometry -- Spectral processing -- Spectral alignment and mass correction -- Baseline correction and noise reduction -- Peak detection and binning -- Background subtraction -- Normalization -- Common and open-source imaging mass spectrometry data analysis -- imzML -- Open-source imaging MS software -- Data analysis functionality for imaging mass spectrometry -- Visualization -- Identification of molecule -- Quantitative imaging measurements -- Multimodal -- Statistical analysis -- Segmentation and clustering -- Conclusions -- References.
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Chapter 11 - Multimodal imaging mass spectrometry -- Image registration -- Multimodal imaging techniques in imaging MS -- Multimodal imaging MS with multiple ion sources -- Multimodal imaging MS with optical microscopy -- Multimodal imaging MS with clinical imaging -- Multimodal imaging MS with other imaging techniques -- Conclusions -- References -- Chapter 12 - Validation and standardization of imaging mass spectrometry -- Validation of imaging MS -- Specificity -- Robustness -- Sensitivity -- Linearity and dynamic range -- Stability -- Accuracy and imprecision -- Postvalidation -- Standardization of imaging MS -- Standardization -- Standardized data format-imzML -- Reporting standards for imaging MS -- Multicenter validation of imaging MS -- Conclusions -- References -- Chapter 13 - Toward clinical imaging mass spectrometry -- Biomedical visualization in clinic -- Histopathology -- Digital pathology -- In vivo imaging -- Labeling in clinical imaging -- Clinical application of imaging MS -- Pathology -- Intraoperative histopathology -- Tissue microarrays -- Treatment response -- Clinical implementation of imaging MS -- Medical device -- Laboratory-developed test -- Outlook -- References -- Chapter 14 - Imaging mass spectrometry: endogenous mammalian metabolites -- Improved sample preparation for metabolite imaging -- Enhancing metabolite detection in MALDI by matrix selection -- Increased mass resolution for metabolite imaging -- Imaging metabolite with ion mobility separation MS -- Using multiple ion sources for metabolite imaging MS -- Outlook -- References -- Chapter 15 - Imaging mass spectrometry: Glycans -- MALDI imaging of glycans -- Targeted glycan imaging -- Outlook -- References -- Chapter 16 - Imaging mass spectrometry: steroids mapping using on-tissue chemical derivatization -- On-tissue chemical derivatization workflow.
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On-tissue chemical derivatization and imaging of steroids -- Outlook -- References -- Chapter 17 - Imaging mass spectrometry: neurotransmitter distribution using reactive matrix and chemical derivatization -- Reactive matrix for imaging neurotransmitters -- On-tissue chemical derivatization for imaging neurotransmitters -- Outlook -- References -- Chapter 18 - Imaging mass spectrometry: small drugs and metabolites in tissue -- Increasing sensitivity for drug imaging by MS -- Increasing selectivity for drug imaging MS -- Outlook -- References -- Chapter 19 - Imaging mass spectrometry: gangliosides in brain tissue -- Matrix selection for ganglioside for MALDI imaging -- Atmospheric pressure MALDI for ganglioside imaging -- Sample preparation for enhanced ganglioside imaging by MS -- Novel developments in ganglioside imaging by MS -- Outlook -- References -- Index -- Back cover.
Weitere Ausg.:
ISBN 9780128189986
Weitere Ausg.:
ISBN 0128189983
Sprache:
Englisch
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