UID:
almafu_9960072210002883
Format:
1 online resource (347 p.)
ISBN:
1-281-75631-8
,
9786611756314
,
0-08-085878-3
Series Statement:
Laboratory techniques in biochemistry and molecular biology ; v. 9
Content:
Sequencing of proteins and peptides
Note:
Description based upon print version of record.
,
Front Cover; Laboratory Techniques in Biochemistry and Molecular Biology; Copyright Page; Contents; Preface; List of abbreviations; Chapter 1. Introduction; 1.1. Protein structure; 1.2. Historical background; 1.3. Outline of the strategy for sequence determination; Chapter 2. Preliminary characterization of the protein; 2.1. Purification of the protein; 2.2. Separation of the constituent polypeptide chains of heterooligomeric proteins; 2.3. Determination of the molecular weight of the polypeptide chains; 2.4. Removal of salts and non-covalently bound molecules; 2.5. Amino acid analysis
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2.6. Chemical modification of the protein for sequence analysis2.7. Determination of the amino-terminal residue; 2.8. Determination of the carboxy-terminal residue; Chapter 3. Specific cleavage of the protein; 3.1. General approach to cleavage; 3.2. Selective cleavage of the polypeptide chain to produce large fragments; 3.3. Enzymic cleavage of denatured polypeptides; 3.4. Specific cleavage of proteins by chemical methods; Chapter 4. Separation and purification of peptides; 4.1. Outline of methods used; 4.2. Amounts of digested protein required
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4.3. Preliminary study of the peptides in a digest4.4. Separation of peptides on the basis of solubility; 4.5. Gel filtration; 4.6. Ion-exchange chromatography; 4.7. Paper electrophoresis; 4.8. Paper chromatography; 4.9. Peptide mapping on paper; 4.10. Thin-layer methods for purification of peptides; 4.11. Preparative polyacrylamide gel electrophoresis; 4.12. High-performance liquid chromatography; 4.13. Affinity chromatography; 4.14. Selective isolation of peptides by covalent binding to an insoluble matrix; 4.15. 'Diagonal' methode
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4.16. Selective purification of blocked N-terminal peptidesChapter 5. Methods for the detection of peptides; 5.1. General methods for the detection of peptides in column eluates; 5.2. General methods for the detection of peptides adsorbed to paper or thin-layer supports; 5.3. Detection methods specific for certain amino acid residues, in column fractions; 5.4. Detection methods specific for certain amino-acid residues, on paper or thin-layer supports; Chapter 6. Determination of peptide sequences; 6.1. Methods for the estimation of the purity of peptides
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6.2. Determination of the N-terminal residues of peptides6.3. Determination of peptide sequences by the Edman degradation; 6.4. Experimental procedures for the Edman degradation; 6.5. Alternative isothiocyanate reagents; 6.6. Other chemical degradation methods for peptide sequence determinations; 6.7. Enzymic methods for peptide sequence determination; 6.8. Mass spectrometry; 6.9. Subfragmentation of peptides; 6.10. Summary of possible difficulties in peptide sequence determination; Chapter 7. Structures introduced by post-translational modification; 7.1. ?-Carboxyglutamic acid
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7.2. Phosphorylated amino-acid residues
Additional Edition:
ISBN 0-444-80275-4
Language:
English
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