UID:
almafu_9960073405702883
Format:
1 online resource (577 p.)
ISBN:
1-281-75963-5
,
9786611759636
,
0-08-085884-8
Series Statement:
Laboratory techniques in biochemistry and molecular biology ; v. 15
Content:
Enzyme immunoassays have developed into a powerful assay technology, transcending several discipline boundaries, extensively applied as a tool in fields other than enzymology and immunology. This volume reflects the rapid progress in the applications of this technique, providing a basic understanding of these techniques and a practical guideline for the choice and experimental detail.
Note:
Description based upon print version of record.
,
Front Cover; Practice and Theory of Enzyme Immunoassays; Copyright Page; Preface; Acknowledgements; Contents; List of abbreviations; Chapter 1. Introduction; 1.1. History of enzyme immunoassays; 1.2. Purpose and organization of this volume; 1.3. The use of terms in enzyme immunoassays; Chapter 2. Outline of the strategies for enzyme immunoassays; 2.1. Classification of enzyme immunoassays; 2.2. Sensitivity and detectability of activity amplification and activity modulation assays; 2.3. Specificity of activity amplification and activity modulation assays
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2.4. General characteristics of enzyme immnunoassay designs2.5. Designs of enzyme immunoassays; Chapter 3. Non-immunologic molecular recognition systems used in immunoassays; 3.1. The avidin/biotin system; 3.2. Preparation of avidin and biotinylated immunoassay reactants; 3.3. Protein A (SPA); 3.4. Lectins: specific carbohydrate-recognizing glycoproteins; Chapter 4. The nature of immunogens, antigens and haptens; Chapter 5. Production of antibodies; 5.1. Biology and regulation of the immune response; 5.2. Production of polyclonal antisera
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5.3. The relative merits of polyclonal and monoclonal antibodies in enzyme immunoassays5.4. Production of monoclonal antibodies; Chapter 6. The nature and structure of antibodies; 6.1. Molecular structure of antibodies; 6.2. Genetic variants of immunoglobulins; 6.3. Genetic basis of antibody diversity; 6.4. Synthesis of antibodies and clonal selection; Chapter 7. Purification of immunoglobulins and preparation of Fab fragments; 7.1. Preparation of immunoglobulins from polyclonal sera; 7.2. Purification of IgY from egg yolk; 7.3. Purification of monoclonal antibodies
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7.4. Assessment of the purity and quality of immunoglobulins7.5. Preparation of Fab fragments; Chapter 8. Kinetics and nature of antibody-antigen interactions; 8.1. Physicochemical basis of antibody-antigen interaction; 8.2. Influence of the pH, ionic strength, temperature, and organic solvents on the stability of the antigen-antibody complex; 8.3. Measurement of the affinity of antibodies; 8.4. Kinetics of antibody-antigen interactions; 8.5. Concept of avidity and its importance in enzyme immunoassays; 8.6. Cross-reactivity, specificity and multispecificity in immunoassays
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8.7. Kinetics of enzyme immunoassaysChapter 9. The nature of enzyme activity in immunoassays; 9.1. Elementary principles of enzyme kinetics; 9.2. Practical details of enzyme catalysis and inhibition; Chapter 10. Properties and preparation of enzymes used in enzyme immunoassays; 10.1. Enzymes used in activity amplification assays; 10.2. Enzymes used in activity modulation assays; 10.3. Enzymes used in enzyme immunohistochemistry; Chapter 11. Preparation of enzyme-antibody or other enzyme-macromolecule conjugates; 11.1. Conjugation procedures and their relative merits
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11.2. Chemical conjugation
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English
Additional Edition:
ISBN 0-444-80634-2
Language:
English
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