UID:
edocfu_9958130212602883
Umfang:
1 online resource (249 p.)
ISBN:
1-281-75988-0
,
9786611759889
,
0-444-82418-9
,
0-08-088696-5
Serie:
Laboratory techniques in biochemistry and molecular biology ; v. 26
Inhalt:
The central role of RNA in many cellular processes, in biotechnology, and as pharmaceutical agents, has created an interest in experimental methods applied to RNA molecules. This book provides scientists with a comprehensive collection of thoroughly tested up-to-date manuals for investigating RNA-protein complexes in vitro. The protocols can be performed by researchers trained in standard molecular biological techniques and require a minimum of specialized equipment. The procedures include recommendation of suppliers of reagents.
Anmerkung:
Description based upon print version of record.
,
Cover; TOCContents; Preface; CHChapter 1. Introduction to RNA-protein interactions; 1.1. Introduction; 1.2. RNA secondary structures; 1.3. RNA tertiary structures; 1.4. Protein motifs involved in RNA binding; References; CHChapter 2. Preparation of RNA; 2.1. Working with RNA; 2.2. Isolation of RNA from cells; 2.3. In vitro synthesis of RNA; References; CHChapter 3. Preparation of protein; 3.1. Isolation of protein from cells; 3.2. Expression and purification of recombinant proteins in E. coli; 3.3. In vitro translation; References
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CHChapter 4. Preparation and analysis of RNA-protein complexes in vitro4.1. Formation of RNA-protein complexes; 4.2. Analysis of RNA-protein complexes; 4.3. Isolation and identification; 4.4. RNA footprinting; 4.5. Protein footprinting; 4.6. Modification interference; 4.7. SELEX; References; CHChapter 5. Functional analysis of RNA-protein complexes in vitro; 5.1. Pre-mRNA splicing; 5.2. Polyadenylation; 5.3. RNA modification; 5.4. Translation; References
,
English
Weitere Ausg.:
ISBN 0-444-82419-7
Sprache:
Englisch
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