Format:
V, 800 S.
,
Ill., graph. Darst.
ISBN:
9780879697723
,
0879697725
,
0879697717
Content:
Plasmids and their usefulness in molecular cloning -- Bacteriophage and its vectors -- Working with bacteriophage M13 -- Working with high-capacity vectors -- Gas electrophoresisof DNA and pulsed-field agarose -- Preparation and analysis of eukaryotic genomic DNA -- Extraction, purification, and analysis of mRNA from eukaryotic cells -- In vitro amplification of DNA by the polymerase chain reaction -- Preparation of radiolabeled DNA and RNA probes -- Working with synthetic oligonucleotide probes -- Preparation of cDNA libraries and gene identification -- DNA sequencing -- Mutagenesis -- Screening expression libraries -- Expression of cloned genes in escherichia coli -- Introducing cloned genes into cultured mammalian cells -- Analysis of gene expression in cultured mammalian cells -- Protein interaction technologies.
Note:
Includes index
Language:
English
Subjects:
Biology
Keywords:
Genklonierung
;
DNS-Klonierung
;
Genklonierung
URL:
http://www.loc.gov/catdir/toc/ecip0517/2005022077.html
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