UID:
almahu_9949251233302882
Umfang:
1 online resource (396 p.)
Ausgabe:
1st ed. 1993.
ISBN:
1-59259-502-2
Serie:
Methods in Molecular Biology, 15
Inhalt:
PCR has been successfully utilized in every facet of basic, cli- cal, and applied studies of the life sciences, and the impact that PCR has had on life science research is already staggering. C- comitant with the essentially universal use of PCR has been the creative and explosive development of a wide range of PCR-based techniques and applications. These increasingly numerous pro- cols have each had the general effect of facilitating and acceler- ing research. Because PCR technology is relatively easy and inexpensive, PCR applications are well within the reach of every research lab. In this sense, PCR has become the "equalizer" between "small" and "big" labs, since its use makes certain projects, especially those related to molecular cloning, now far more feasible for the small lab with a modest budget. This new volume on PCR Protocols does not attempt the impossible task of representing all PCR-based protocols. Rather, it presents a range of protocols, both analytical and preparative, that provide a solid base of knowledge on the use of PCR in many c- mon research problems. The first six chapters provide some basic information on how to get started. Chapters 7-19 represent primarily analytical uses of PCR, both for simple DNA and RNA detection, as well as for more complex analyses of nucleic acid (e. g. , DNA footprin ting, RNA splice site localization). The remaining chapters represent "synthetic," or preparative, uses of PCR.
Anmerkung:
Description based upon print version of record.
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Polymerase Chain Reaction -- Selection of Primers for Polymerase Chain Reaction -- Direct Radioactive Labeling of Polymerase Chain Reaction Products -- Use of Arithmetic Polymerase Chain Reaction for Synthesis of Single-Stranded Probes for S1 Nuclease Assays -- Nonradioactive Labeling of Polymerase Chain Reaction Products -- Quantitation and Purification of Polymerase Chain Reaction Products by High-Performance Liquid Chromatography -- Use of Polymerase Chain Reaction for Screening Transgenic Mice -- Polymerase Chain Reaction Analysis of DNA from Paraffin-Embedded Tissue -- The Use of Polymerase Chain Reaction for Chromosome Assignment -- Mapping MHC Class II Genes and Disease-Susceptibility -- The Use of the Polymerase Chain Reaction and the Detection of Amplified Products -- Determination of Loss of Heterozygosity Using Polymerase Chain Reaction -- Direct Sequencing of Polymerase Chain Reaction Products -- Manual and Automated Direct Sequencing of Product Generated by the Polymerase Chain Reaction -- Genomic Footprinting by Ligation Mediated Polymerase Chain Reaction -- RNA Template-Specific Polymerase Chain Reaction (RS-PCR) -- Quantitative Measurement of Relative Gene Expression in Human Tumors -- Identification of Alternatively Spliced mRNAs and Localization of 5' Ends by Polymerase Chain Reaction Amplification -- Utilization of Polymerase Chain Reaction for Clonal Analysis of Gene Expression -- Sequencing DNA Amplified Directly from a Bacterial Colony -- Use of Polymerase Chain Reaction to Screen Phage Libraries -- Molecular Cloning of Polymerase Chain Reaction Fragments with Cohesive Ends -- Rapid (Ligase-Free) Subcloning of Polymerase Chain Reaction Products -- Use of Polymerase Chain Reaction for Making Recombinant Constructs -- In Vitro Recombination and Mutagenesis of DNA -- Use of Polymerase Chain Reaction for the Rapid Construction of Synthetic Genes -- Recombinant Circle Polymerase Chain Reaction for Site-Directed Mutagenesis -- Site-Directed Mutagenesis by Double Polymerase Chain Reaction -- Generation of a Polymerase Chain Reaction Renewable Source of Subtractive cDNA -- PCR-Based Full-Length cDNA Cloning Utilizing the Universal-Adaptor/Specific DOS Primer-Pair Strategy -- Use of Degenerate Oligonucleotide Primers and the Polymerase Chain Reaction to Clone Gene Family Members -- Single Specific Primer-Polymerase Chain Reaction (SSP-PCR) and Genome Walking -- cDNA Cloning by Inverse Polymerase Chain Reaction -- Amplification of Gene Ends from Gene Libraries by Polymerase Chain Reaction with Single-Sided Specificity -- Anchoring a Defined Sequence to the 5? Ends of mRNAs.
,
English
Weitere Ausg.:
ISBN 0-89603-244-2
Sprache:
Englisch
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