Format:
Online-Ressource (VII, 174 S.)
,
Ill.
Edition:
2015
ISBN:
9783110426250
,
9783110411324
,
9783110411331
Content:
Main description: Biochemistry Laboratory Manual for undergraduates is the first textbook on the market that uses a highly relevant model, antibiotic resistance, to teach seminal topics of biochemistry and molecular biology. Inclusion of a research project does not entail a limitation: this manual includes all classic biochemistry techniques such as HPLC or enzyme kinetics and is complete with numerous problem sets relating to each topic.
Content:
Biographical note: Timea Gerczei Fernandez, Ball State University, Muncie, USAScott Patison, Ball State University, Muncie, USA
Note:
FrontmatterPrefaceContents1 Introducing the Bacterial Antibiotic Sensor Mini Project2 Identifying Conserved Elements in the Toxin Sensor and Designing Mutants to Test Whether They are Important for Function3 Designing Primers for Site-Directed Mutagenesis4 Performing Site-Directed Mutagenesis5 Purifying Mutant Toxin Sensor DNA from Bacterial Cells and Evaluating its Quality Using Agarose Gel Electrophoresis and UV Spectroscopy6 Preparing DNA Template for Mutant RNA Sensor Synthesis Using a Restriction Endonuclease7 Synthesizing the ykkCD Mutant Toxin Sensor RNA in vitro8 Purifying the ykkCD Mutant Toxin Sensor RNA and Evaluating its Purity Using Denaturing PAGE and UV spectrometry9 Evaluating the Ability of the ykkCD Toxin Sensor to Recognize the Antibiotic Tetracycline Using Fluorescent Quenching10 Evaluating Antibiotic Binding to Blood Serum Albumin Using Fluorescence Spectroscopy11 Understanding the Importance of Buffers in Biological Systems12 Molecular Visualization of an Enzyme, Acetylcholinesterase13 Determining the Efficiency of the Enzyme Acetylcholine Esterase Using Steady-State Kinetic Experiment14 Separation of the Phosphatidylcholines Using Reverse Phase HPLCList of Figures.
Language:
English
Keywords:
Biochemie
;
Labortechnik
DOI:
10.2478/9783110411331
URL:
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