UID:
almafu_9958129886302883
Umfang:
1 online resource (475 p.)
ISBN:
9786611011550
,
9781281011558
,
128101155X
,
9780080497266
,
0080497268
Serie:
Methods in enzymology ; v. 389
Inhalt:
This volume is an in-depth treatment of G-Protein Signaling, Part A, and will cover general methods of analysis of RGS protein analysis, including Expression and post-translational modification, Assays of GAP activity and allosteric control, Electrophysiological methods and RGS-insensitive Ga subunits, Mouse models of RGS protein action, Methods of RGS protein inhibition, and G-protein regulators of model organisms.Table of Contents-Expression and post-translational modification-Assays of GAP activity and allosteric control-Electrophysiological methods and RGS-insenstitive
Anmerkung:
Description based upon print version of record.
,
Cover; Table of Contents; Contributors to Volume 389; Preface; Volumes in Series; Section I: General Methods of RGS Protein Analysis Subsection A. Expression and Post-translational Modification; Chapter 1. Quantitative Real-Time Polymerase Chain Reaction Measurement of Regulators of G-Protein Signaling mRNA Levels in Mouse Tissues; Chapter 2. Regulation of Chemokine-Induced Lymphocyte Migration by RGS Proteins; Chapter 3. Role of Palmitoylation in RGS Protein Function; Subsection B: Assays of RGS Box Activity and Allosteric Control; Chapter 4. Fluorescence-Based Assays for RGS Box Function
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Chapter 5. Application of RGS Box Proteins to Evaluate G-Protein Selectivity in Receptor-Promoted SignalingChapter 6. Allosteric Regulation of GAP Activity by Phospholipids in Regulators of G-Protein Signaling; Chapter 7. Assays of RGS Protein Modulation by Phosphatidylinositides and Calmodulin; Subsection C. Electrophysiological Methods and RGS-Insensitive Galpha Subunits; Chapter 8. Analyses of RGS Protein Control of Agonist- Evoked Ca2+ Signaling; Chapter 9. Measuring the Modulatory Effects of RGS Proteins on GIRK Channels
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Chapter 10. Assays for G-Protein-Coupled Receptor Signaling Using RGS-Insensitive Galpha SubunitsChapter 11. Use of RGS-Insensitive Galpha Subunits to Study Endogenous RGS Protein Action on G-Protein Modulation of N-Type Calcium Channels in Sympathetic Neurons; Chapter 12. Endogenous RGS Proteins Regulate Presynaptic and Postsynaptic Function: Functional Expression of RGS-Insensitive Galpha Subunits in Central Nervous System Neurons; Subsection D. Mouse Models of RGS Protein Action
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Chapter 13. In Situ Hybridization Analysis of RGS mRNA Regulation and Behavioral Phenotyping of RGS Mutant MiceChapter 14. RGS-Insensitive G-Protein Mutations to Study the Role of Endogenous RGS Proteins; Subsection E. Methods of RGS Protein Inhibition; Chapter 15. Ribozyme- and siRNA-Mediated Suppression of RGS-Containing RhoGEF Proteins; Chapter 16. Structure-Based Design, Synthesis, and Activity of Peptide Inhibitors of RGS4 GAP Activity; Chapter 17. Yeast-Based Screening for Inhibitors of RGS Proteins; Section II: G-Protein Regulators of Model Organisms
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Chapter 18. Genetic Analysis of RGS Protein Function in Caenorhabditis elegansChapter 19. Purification and In Vitro Functional Analysis of the Arabidopsis thaliana Regulator of G-Protein Signaling-1; Chapter 20. AtRGS1 Function in Arabidopsis thaliana; Chapter 21. Identification and Functional Analysis of the Drosophila Gene loco; Chapter 22. Analysis of the Roles of Pins and Heterotrimeric G Proteins in Asymmetric Division of Drosophila Neuroblasts; Chapter 23. Mathematical Modeling of RGS and G-Protein Regulation in Yeast
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Chapter 24. Identification of Yeast Pheromone Pathway Modulators by High-Throughput Agonist Response Profiling of a Yeast Gene Knockout Strain Collection
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English
Weitere Ausg.:
ISBN 9780121827946
Weitere Ausg.:
ISBN 0121827941
Sprache:
Englisch
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