UID:
almafu_9958132550202883
Umfang:
1 online resource (583 p.)
ISBN:
9786611011031
,
9781281011039
,
1281011037
,
9780080496740
,
0080496741
Serie:
Methods in enzymology ; v. 317
Inhalt:
RNA-Ligand Interactions, Part A focuses on structural biology methods. Major topics covered include semisynthetic methodologies (RNA synthetic methods and derivatization of RNA); RNA structure determination (X-ray crystallography, NMR, EM); techniques for monitoring RNA conformation and dynamics (solution methods and electrophoretic and spectroscopic methods); and modeling tertiary structure: Part B, its companion Volume 318 of Methods in Enzymology, focuses on molecular biology methods.The critically acclaimed laboratory standard for more than forty years, Methods
Anmerkung:
Description based upon print version of record.
,
Front Cover; Methods in Enzymology; Copyright Page; Table of Contents; Contributors to Volume 317; Preface; Volumes in Series; Section I: Semisynthetic Methodologies; A. RNA Synthetic Methods; Chapter 1. Advanced 5' -Silyl-2' -Orthoester Approach to RNA Oligonucleotide Synthesis; Chapter 2. Preparation of Specifically 2H- and 13C-Labeled Ribonucleotides; Chapter 3. Base-Modified Phosphoramidite Analogs of Pyrimidine Ribonucleosides for RNA Structure- Activity Studies; B. Derivatization of RNA; Chapter 4. Use of T7 RNA Polymerase and Its Mutants for Incorporation of Nucleoside Analogs into RNA
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Chapter 5. Phosphorothioate Modification of RNA for Stereochemical and Interference AnalysesChapter 6. Chemical Probing of RNA by Nucleotide Analog Interference Mapping; Chapter 7. Joining of RNAs by Splinted Ligation; Chapter 8. Heavy Atom Derivatives of RNA; Chapter 9. Site-Specific Cleavage of Transcript RNA; Chapter 10. Using DNAzymes to Cut, Process, and Map RNA Molecules for Structural Studies or Modification; Section II: RNA Structure Determination; A. X-Ray Crystallography; Chapter 11. Purification, Crystallization, and X-Ray Diffraction Analysis of Small Ribozymes
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Chapter 12. Solving Large RNA Structures by X-Ray CrystallographyChapter 13. Conventional and Time-Resolved Ribozyme X-Ray Crystallography; B. Nuclear Magnetic Resonance Spectroscopy; Chapter 14. Nuclear Magnetic Resonance Methods to Study RNA-Protein Complexes; Chapter 15. Filamentous Bacteriophage for Aligning RNA, DNA, and Proteins for Measurement of Nuclear Magnetic Resonance Dipolar Coupling Interactions; Chapter 16. Biochemical and Nuclear Magnetic Resonance Studies of Aminoglycoside-RNA Complexes; C. Electron Microscopy
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Chapter 17. Experimental Prerequisites for Determination of tRNA Binding to Ribosomes from Escherichia coliChapter 18. Three-Dimensional Cryoelectron Microscopy of Ribosomes; Chapter 19. Preparation of Functional Ribosomal Complexes and Effect of Buffer Conditions on tRNA Positions Observed by Cryoelectron Microscopy; Section III: Techniques for Monitoring RNA Conformation and Dynamics; A. Solution Methods; Chapter 20. Probing RNA Structure and Function by Circular Permutation; Chapter 21. Kinetic Oligonucleotide Hybridization for Monitoring Kinetic Folding of Large RNAs
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Chapter 22. Time-Resolved Synchrotron X-Ray Footprinting and Its Application to RNA FoldingB. Electrophoretic and Spectroscopic Methods; Chapter 23. Analysis of Global Conformation of Branched RNA Species Using Electrophoresis and Fluorescence; Chapter 24. Application of Circular Dichroism to Study RNA Folding Transitions; Chapter 25. Fluorescence Assays to Study Structure, Dynamics, and Function of RNA and RNA-Ligand Complexes; Chapter 26. Transient Electric Birefringence for Determining Global Conformations of Nonhelix Elements and Protein-Induced Bends in RNA
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Section IV: Modeling Tertiary Structure
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English
Weitere Ausg.:
ISBN 9780121822187
Weitere Ausg.:
ISBN 0121822184
Sprache:
Englisch
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