UID:
almafu_9958132552302883
Umfang:
1 online resource (603 p.)
ISBN:
9786611011062
,
9781281011060
,
1281011061
,
9780080496771
,
0080496776
Serie:
Methods in enzymology ; v. 322
Inhalt:
Volume 322 of Methods in Enzymology is dedicated to apoptosis. Major topics covered include measuring apoptosis and apoptosis-induced endonucleases, measuring apoptosis in lower organisms, proteases involved in apoptosis and their inhibitors, cell free systems for monitoring steps in apoptosis pathways, mitochondria and apoptosis, bCl-2 family proteins, and studying receptors and signal transduction events implicated in cell survival and cell death.The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly re
Anmerkung:
Description based upon print version of record.
,
Front Cover; Apoptosis; Copyright Page; Table of Contents; Contributors to Volume 322; Preface; Volumes in Series; Section I: Measuring Apoptosis and Apoptosis-Induced Endonucleases; Chapter 1. Detection of DNA Cleavage in Apoptotic Cells; Chapter 2.Detection of Apoptosis by Annexin V Labeling; Chapter 3. Analysis of Apoptotic Cells by Flow and Laser Scanning Cytometry; Chapter 4. Quantitative Measurement of Apoptosis Induced by Cytotoxic T Lymphocytes; Chapter 5. Apoptotic Nuclease Assays; Section II: Measuring Apoptosis in Lower Organisms
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Chapter 6. Analysis of Programmed Cell Death and Apoptosis in DrosophilaChapter 7. Programmed Cell Death in the Nematode Caenorhabditis elegans; Section III: Proteases Involved in Apoptosis and Their Inhibitors; Chapter 8. Caspase Assays; Chapter 9. Determination of Caspase Specificities Using a Peptide Combinatorial Library; Chapter 10. Criteria for Identifying Authentic Caspase Substrates during Apoptosis; Chapter 11. Purification and Use of Granzyme B; Chapter 12. Viral Caspase Inhibitors CrmA and p35
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Chapter 13. Purification and Use of Recombinant Inhibitor of Apoptosis Proteins as Caspase InhibitorsChapter 14. Monitoring Activity of Caspases and Their Regulators in Yeast Saccharomyces cerevisiae; Section IV: Cell-Free Systems for Monitoring Steps in Apoptosis Pathways; Chapter 15. In Vitro Assays for Caspase-3 Activation and DNA Fragmentation; Chapter 16. Cell-Free Apoptosis in Xenopus laevis Egg Extracts; Chapter 17. Cytofluorometric Quantification of Nuclear Apoptosis Induced in a Cell-Free System; Section V: Mitochondria and Apoptosis
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Chapter 19. Quantitation of Mitochondrial Transmembrane Potential in Cells and in Isolated MitochondriaChapter 20. Nitrogen Cavitation for Cell Disruption to Obtain Mitochondria from Cultured Cells; Chapter 21. Apoptosis-Related Activities Measured with Isolated Mitochondria and Digitonin-Permeabilized Cells; Chapter 22. Assays for Cytochrome c Release from Mitochondria during Apoptosis; Chapter 23. Purification and Liposomal Reconstitution of Permeability Transition Pore Complex; Section VI: Bcl-2 Family Proteins; Chapter 18. Purification of Mitochondria for Apoptosis Assays
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Chapter 24. Monitoring Interactions of Bcl-2 Family Proteins in 96-Well Plate AssaysChapter 25. Analysis of Dimerization of Bcl-2 Family Proteins by Surface Plasmon Resonance; Chapter 26. Measuring Pore Formation by Bcl-2 Family Proteins; Chapter 27. Assays for Studying Bax-Induced Lethality in the Yeast Saccharomyces cerevisiae; Chapter 28. Exploiting the Utility of Yeast in the Context Programmed Cell Death; Section VII: Studying Receptors and Signal Transduction Events Implicated in Cell Survival and Cell Death
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Chapter 29. Production of Recombinant TRAIL and TRAIL Receptor: Fc Chimeric Proteins
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English
Weitere Ausg.:
ISBN 9780121822231
Weitere Ausg.:
ISBN 0121822230
Sprache:
Englisch
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