UID:
almafu_9958132553802883
Umfang:
1 online resource (740 p.)
ISBN:
9786611011192
,
9781281011190
,
1281011193
,
9780080496900
,
0080496903
Serie:
Methods in enzymology ; v. 340
Inhalt:
This volume consolidates the key methods for studying ligand-nucleic acid interactions into a convenient source. Techniques that are examined range from biophysical and chemical approaches to methods rooted in molecular and cell biology.
Anmerkung:
Description based upon print version of record.
,
Front Cover; Drug-Nucleic Acid Interactions; Copyright Page; Table of Contents; Contributors to Volume 340; Preface; Volume in Series; Section I: Biophysical Approaches; Chapter 1. Analysis and Interpretation of Ligand-DNA Binding Isotherms; Chapter 2. Surface Plasmon Resonance Biosensor Analysis of RNA-Small Molecule Interactions; Chapter 3. Simultaneous Measurement of Binding Constants and Unwinding Angles by Gel Electrophoresis; Chapter 4. Linear and Circular Dichroism of Drug-Nucleic Acid Complexes; Chapter 5. Rapid Screening of Structurally Selective Ligand Binding to Nucleic Acids
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Chapter 6. Calorimetric Techniques in the Study of High-Order DNA-Drug InteractionsChapter 7. Volume Changes Accompanying Interaction of Ligands with Nucleic Acids; Chapter 8. Calculating Sequence-Dependent Melting Stability of Duplex DNA Oligomers and Multiplex Sequence Analysis by Graphs; Chapter 9. Thermal Denaturation as Tool to Study DNA-Ligand Interactions; Chapter 10. Kinetics of Binding of Hoechst Dyes to DNA Studied by Stopped-Flow Fluorescence Techniques; Chapter 11. Scanning Force Microscopy of Nucleic Acid Complexes
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Chapter 12. Nuclear Magnetic Resonance Studies of Drug-DNA Complexes in SolutionChapter 13. X-Ray Crystallography of DNA-Drug Complexes; Chapter 14. Molecular Modeling of Drug-DNA Complexes: An Update; Section II: Chemical and Molecular Biological Approaches; Chapter 15. Peptide Nucleic Acid Targeting of Double-Stranded DNA; Chapter 16. Drug Interaction with Triple-Helical Nucleic Acids; Chapter 17. Measurement of Covalent Drug-DNA Interactions at the Nucleotide Level in Cells at Pharmacologically Relevant Doses
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Chapter 18. Targeting DNA through Covalent Interactions of Reversible Binding DrugsChapter 19. Chemical Cross-Linking of Drugs to DNA; Chapter 20. High-Resolution Footprinting Studies of Drug-DNA Complexes Using Chemical and Enzymatic Probes; Chapter 21. Drug-RNA Footprinting; Chapter 22. Footprinting Methods for Analysis of Pyrrole-Imidazole Polyamide/DNA Complexes; Chapter 23. High-Resolution Transcription Assay for Probing Drug-DNA Interactions at Individual Drug Sites; Chapter 24. Use of DNA Molecules Substituted with Unnatural Nucleotides to Probe Speci.c Drug-DNA Interactions
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Chapter 25. Drug Interactions with Nucleosomes and ChromatinChapter 26. Locating Cobalt-Binding Sites on DNA Using Restriction Endonucleases; Chapter 27 Exploiting Anthracycline Scaffold for Designing DNA-Targeting Agents; Chapter 28. Design, Synthesis, and Characterization of Polyintercalating Ligands; Section III: Enzymology and Biological Approaches; Chapter 29. Targeting Telomeres and Telomerase; Chapter 30. Rapid, High-Throughput Engineering of Sequence-Specific Zinc Finger DNA-Binding Proteins; Chapter 31. DNA Relaxation and Cleavage Assays to Study Topoisomerase I Inhibitors
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Chapter 32. In Vitro Human Immunodeficiency Virus Type 1 Integrase Assays
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English
Weitere Ausg.:
ISBN 9780121822415
Weitere Ausg.:
ISBN 0121822419
Sprache:
Englisch
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