UID:
almafu_9958132554002883
Umfang:
1 online resource (555 p.)
ISBN:
9786611020859
,
9781281020857
,
1281020850
,
9780080522579
,
0080522572
Serie:
Methods in enzymology ; v. 342, Part B
Inhalt:
This second volume on ribonucleases provides up-to-date, methods-related information on these enzymes. Of particular interest to researchers will be the discussion of artificial and engineered ribonucleases, as well as the application of ribonucleases in medicine and biotechnology.The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more
Anmerkung:
Description based upon print version of record.
,
Front Cover; Ribonucleases; Copyright Page; Table of Contents; Contributors to Volume 342; Preface; Volume in Series; Section I: Processing and Degradative Endoribonucleases; Chapter 1. Purification and Activity Assays of the Catalytic Domains of the Kinase/Endoribonuclease Ire1p from Saccharomyces; Chapter 2. Monitoring Activation of Ribonuclease L by 2 ́, 5 ́-Oligoadenylates Using Purified Recombinant Enzyme and Intact Malig; Chapter 3. Accelerating RNA Decay through Intervention of RNase L: Alternative Synthesis of Composite 2 ́,5 ́-Oligoadenylate-Antisense; Chapter 4. Polysomal Ribonuclease 1
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Chapter 5. Liver Perchloric Acid-Soluble RibonucleaseChapter 6. Escherichia coli Ribonuclease G; Chapter 7. Escherichia coli Transcript Cleavage Factors GreA and GreB: Functions and Mechanisms of Action; A. Ribonucleoprotein Ribonucleases; Chapter 8. Escherichia coli Ribonuclease P; Chapter 9. Human Ribonuclease P; Chapter 10. Saccharomyces cerevisiae Nuclear Ribonuclease P: Structure and Function; Chapter 11. Cyanelle Ribonuclease P: Isolation and Structure-Function Studies of an Organellar Ribonucleoprotein Enzyme; Chapter 12. Characterization of Ribonuclease MRP Function
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B. Double-Strand-Specific RibonucleasesChapter 13. Escherichia coli Ribonuclease III: Affinity Purification of Hexahistidine-Tagged Enzyme and Assays for Substrate Binding and Cleavage; Chapter 14. Purification and Characterization of Saccharomyces cerevisiae Rnt1p Nuclease; Chapter 15. Pac1 Ribonuclease of Schizosaccharomyces pombe; Chapter 16. Dictyostelium Double-Stranded Ribonuclease; Chapter 17. Double-Stranded RNA Nuclease Associated with Rye Germ Ribosomes; C. Ribonucleases That Cleave Atypical Phosphodiesters; Chapter 18. Yeast mRNA Decapping Enzyme
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Chapter 19. RNA Lariat Debranching EnzymeSection II: Processing and Degradative Exoribonucleases; A. 5 ́-〉3 ́-Exoribonuclease; Chapter 20. 5 ́-Exoribonuclease 1: Xrn1; Chapter 21. Rat1p Nuclease; Chapter 22. Analysis of XRN Orthologs by Complementation of Yeast Mutants and Localization of XRN-GFP Fusion Proteins; Chapter 23. 5 ́-〉3 ́-Exoribonuclease from Rabbit Reticulocytes; Chapter 24. Drosophila 5 ́-〉3 ́-Exoribonuclease Pacman; B. 3 ́-〉5 ́-Exoribonuclease; Chapter 25. Purification of Poly(A)-Specific Ribonuclease; Chapter 26. Escherichia coli Ribonuclease II; Section III: Ribonuclease Complexes
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Chapter 27. Escherichia coli RNA DegradosomeChapter 28. Preparation of Escherichia coli Rne Protein and Reconstitution of RNA Degradosome; Chapter 29. Purification of Yeast Exosome; Section IV: Organellar Ribonucleases; Chapter 30. Genetic and Biochemical Approaches for Analysis of Mitochondrial Degradosome from Saccharomyces cerevisiae; Chapter 31. Direct Sizing of RNA Fragments Using RNase- Generated Standards; Chapter 32. Chlamydomonas reinhardtii as a Model System for Dissecting Chloroplast RNA Processing and Decay Mechanisms; Chapter 33. Chloroplast mRNA 3 ́-End Nuclease Complex
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Chapter 34. Chloroplast p54 Endoribonuclease
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English
Weitere Ausg.:
ISBN 9780121822439
Weitere Ausg.:
ISBN 0121822435
Sprache:
Englisch
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