Umfang:
Online-Ressource (XL, 403, [20] S.)
Ausgabe:
Online-Ausg. 2009 Electronic reproduction; Available via World Wide Web
ISBN:
0121828174
,
9780121828172
Serie:
Methods in enzymology 412
Inhalt:
The ability of polypeptides to form alternatively folded, polymeric structures such as amyloids and related aggregates is being increasingly recognized as a major new frontier in protein research. This new volume of Methods in Enzymologyalong with Part C (volume 413) on Amyloid, Prions and other Protein Aggregates continue in the tradition of the first volume (309) in containing detailed protocols and methodological insights, provided by leaders in the field, into the latest methods for investigating the structures, mechanisms of formation, and biological activities of this important class of protein assemblies. * Presents detailed protocols * Includes troubleshooting tips * Provides coverage on structural biology, computational methods, and biology
Anmerkung:
Literaturangaben
,
Cover; Title Page; Copyright Page; Table of Contents; Contributors to Volume 412; Preface; Volumes in Series; Section I: Characterization of Protein Deposition In Vivo and Ex Vivo; Chapter 1. Protein Misfolding Cyclic Amplification for Diagnosis and Prion Propagation Studies; Chapter 2. Fractionation of Prion Protein Aggregates by Asymmetrical Flow Field-Flow Fractionation; Chapter 3. Analysis of Amyloid Aggregates Using Agarose Gel Electrophoresis; Chapter 4. Characterization of Systemic Amyloid Deposits by Mass Spectrometry; Chapter 5. Proteomics of Polyglutamine Aggregates
,
Chapter 6. Merger of Laser Capture Microdissection Mass Spectrometry: A Window into the Amyloid Plaque ProteomeChapter 7. MALDI MS Imaging of Amyloid; Chapter 8. Imaging Polyglutamine Deposits in Brain Tissue; Chapter 9. X-34 Labeling of Abnormal Protein Aggregates During the Progression of Alzheimer's Disease; Chapter 10. Visualizing Pathology Deposits in the Living Brain of Patients with Alzheimer's Disease; Chapter 11. Micro-Imaging of Amyloid in Mice; Section II: Cell and Animal Models of Amyloid Formation and Toxicity
,
Chapter 12. An Ef.cient Protein Transformation Protocol for Introducing Prions into YeastChapter 13. Screening for Genetic Modifiers of Amyloid Toxicity in Yeast; Chapter 14. Searching for Anti-Prion Compounds: Cell-Based High-Throughput In Vitro Assays and Animal Testing Strategies; Chapter 15. A Drosophila Model of Alzheimer's Disease; Chapter 16. Modeling Polyglutamine Pathogenesis in C. elegans; Section III: Computational Approaches and Theory; Chapter 17. Nucleation: The Connections Between Equilibrium and Kinetic Behavior
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Chapter 18. Amyloid Fibril Structure Modeling Using Protein Threading and Molecular Dynamics SimulationsChapter 19. Ab initio Discrete Molecular Dynamics Approach to Protein Folding and Aggregation; Chapter 20. Computational Approaches to Fibril Structure and Formation; Author Index; Subject Index
,
Electronic reproduction; Available via World Wide Web
In:
Part B
Weitere Ausg.:
Erscheint auch als Druck-Ausgabe Amyloid, prions, and other protein aggregates ; Part B: Amyloid, prions, and other protein aggregates Amsterdam [u.a.] : Elsevier, 2006 ISBN 0121828174
Weitere Ausg.:
ISBN 9780121828172
Sprache:
Englisch
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