UID:
almafu_9958129888302883
Umfang:
1 online resource (736 p.)
ISBN:
9786610751419
,
9781280751417
,
128075141X
,
9780080468983
,
0080468985
Serie:
Methods in enzymology, volume 414
Inhalt:
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today-truly an essential publication for researchers in all fields of life sciences.
Anmerkung:
Description based upon print version of record.
,
Front Cover; Measuring Biological Responses with Automated Microscopy; Copyright Page; Table of Contents; Contributors to Volume 414; Preface; Volumes in Series; Chapter 1: Dynamic Green Fluorescent Protein Sensors for High- Content Analysis of the Cell Cycle; Introduction; Design and Construction of Dynamic Cell Cycle Sensors; Validation of Sensors; Cell Cycle Analysis by Automated High-Throughput Imaging for Drug Profiling and Target Validation; Conclusions and Future Directions; Acknowledgments; References; Chapter 2: High-Content Fluorescence-Based Screening for Epigenetic Modulators
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IntroductionEpigenetic Regulators of Gene Expression as Drug Targets; Rationale for the Development of Cell-Based Assays to Screen for Epigenetic Modulators; Methodological Considerations; General Conclusions and Perspectives; References; Chapter 3: Development of Assays for Nuclear Receptor Modulators Using Fluorescently Tagged Proteins; Introduction; Methods; Perspectives and Future Applications; References; Chapter 4: The Ligand-Independent Translocation Assay: An Enabling Technology for Screening Orphan G Protein-Coupled Receptors by Arrestin Recruitment; Introduction
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Overview of the LITe AssayUtility of the LITe Assay; Conclusion; Acknowledgments; References; Chapter 5: High-Content Screening of Known G Protein-Coupled Receptors by Arrestin Translocation; Introduction; Stable Expression of a Known GPCR for the ArrestinGFP Translocation Assay; Screening Known Receptors with Transfluor Technology; Conclusion; Acknowledgments; References; Chapter 6: Cell Imaging Assays for G Protein-Coupled Receptor Internalization: Application to High-Throughput Screening; Introduction; Monitoring GPCR Trafficking via Receptor-GFP
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Monitoring GPCR Trafficking via Receptor-Arrestin-GFPCase I: MRG-X1 Receptor; Case Study 2: NK1 Receptor Trafficking; Acknowledgments; References; Chapter 7: High-Throughput Confocal Microscopy for ß-Arrestin-Green Fluorescent Protein Translocation G Protein-coupled Receptor Assays Using the Evotec Opera; Choice of Orphans; Transfluor; Technology; Image Analysis Algorithm; EC50 Fitting and Scoring; Assay Methods; Screen Metrics and Results; Concluding Remarks; References; Chapter 8: G Protein-Coupled Receptor Internalization Assays in the High-Content Screening Format; Introduction
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High-Throughput Confocal Cellular Imaging SystemsGPCR Internalization Assays; Internalization Assay and Image Analysis Protocols; Conclusions and Outlook; Acknowledgment; References; Chapter 9: Screening for Activators of the Wingless Type/Frizzled Pathway by Automated Fluorescent Microscopy; Introduction; Use of Primary Human Preosteoblasts; Reagents and Materials; Preparation of L Cell Control- and Wnt3A-Conditioned Medium; Primary Preosteoblast Cell Culture and Compound Screening; Immunofluorescent Staining; Imaging and Quantitation of Nuclear Translocation; Data Analysis; Acknowledgments
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References
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English
Weitere Ausg.:
ISBN 9780121828196
Weitere Ausg.:
ISBN 0121828190
Sprache:
Englisch
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