UID:
almafu_9958129773002883
Umfang:
1 online resource (629 p.)
ISBN:
9786611118976
,
9781281118974
,
1281118974
,
9780080548708
,
0080548709
Serie:
Methods in cell biology ; v. 83
Inhalt:
Cell mechanics is the field of study that looks at how cells detect, modify, and respond to the physical properties of the cell environment. Cells communicate with each other through chemical and physical signals which are involved in a range of process from embryogenesis and wound healing to pathological conditions such as cancerous invasion. Similar principles are also likely to be critical for success in regenerative medicine. Cell mechanics is thus central to understanding these principles. As cell mechanics draws from the fields of biology, chemistry, physics, engineering, and mathemati
Anmerkung:
Description based upon print version of record.
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Front Cover; Methods in Cell Biology; Copyright Page; Contents; Contributors; Preface; Part I: Basic Concept and Preparation Culture Substrates for Cell Mechanical Studies; Chapter 1: Basic Rheology for Biologists; I. Introduction and Rationale; II. Rheological Concepts; A. Elasticity; B. Viscosity; C. Oscillatory Measurements; III. Rheological Instrumentation; IV. Experimental Design; A. Stress-Strain Relation; B. Stress Relaxation; C. Creep and Creep Recovery; D. Frequency Sweep; E. Time Sweep; F. Strain or Stress Amplitude Sweeps; G. Rate-Dependent Viscosity; H. Flow Oscillation
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V. Sample PreparationA. Solids; B. Liquids and Gelling Systems; VI. Special Considerations for Biological Samples; A. Biological Polymers; B. Intact Tissue; C. Instrument Selection for Measuring Gelation Kinetics; VII. Conclusions; Glossary; References; Chapter 2: Polyacrylamide Hydrogels for Cell Mechanics: Steps Toward Optimization and Alternative Uses; I. Introduction; II. Principle of the Polyacrylamide Hydrogel; A. Standard Method of Polymerization; B. Light-Induced Initiation of Polymerization; III. Conjugation of Proteins to Polyacrylamide; A. Carbodiimide-Mediated Cross-Linking
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B. Activation with Acrylic Acid N-Hydroxysuccinimide EsterC. Activation with the N-Succinimidyl Ester of Acrylamidohexanoic Acid (N6); D. Other Protein-Coupling Methods; IV. Optimizing the Placement of Beads for Traction Force Microscopy; V. Manipulation of Gel Geometry; A. Preparation of Polyacrylamide Microbeads; B. Preparation of a Model Three-Dimensional Culture System; VI. Concluding Remarks; References; Chapter 3: Microscopic Methods for Measuring the Elasticity of Gel Substrates for Cell Culture: Microspheres, Microindenters, and Atomic Force Microscopy; I. Introduction
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II. Probing with Microspheres Under Gravitational ForcesIII. Atomic Force Microscopy; IV. Probing with Spherically Tipped Glass Microindenters; A. Preparation and Calibration of the Spherically Tipped Microindenter; B. Calibration of the Microscope and Micromanipulator; C. Characterization and Calibration of the Microindenter; D. Measurement of the Indentations of Hydrogels in Response to Forces of the Microindenter; E. Data Analysis; F. Discussion; V. Conclusions; References; Chapter 4: Surface Patterning; I. Introduction; II. Patterning with Electrodynamic Instabilities
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A. Procedure for Patterning with Electrohydrodynamic InstabilitiesB. Cell Migration on Topographic Surfaces; III. Lithography Without a Clean Room; A. Photolithography Basics; IV. Patterning at the Micro- and Nanoscale with Polymer Mixtures and Block Copolymers; A. Principles and Procedures for Controlling Pattern Formation with Polymer Mixtures and Block Copolymers; V. Summary; Acknowledgments; References; Chapter 5: Molecular Engineering of Cellular Environments: Cell Adhesion to Nano-Digital Surfaces; I. Introduction: Sensing Cellular Environments
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II. Nano-Digital Chemical Surfaces for Regulating Transmembrane-Receptor Clustering
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English
Weitere Ausg.:
ISBN 9780123705006
Weitere Ausg.:
ISBN 0123705002
Sprache:
Englisch
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