UID:
almafu_9958128519302883
Umfang:
1 online resource (431 p.)
ISBN:
9786610962914
,
9781280962912
,
1280962917
,
9780080471129
,
0080471129
Serie:
Methods in enzymology, v. 418
Inhalt:
This is the first of three planned volumes in the Methods in Enzymology series on the topic of stem cells. This volume is a unique anthology of stem cell techniques written by experts from the top laboratories in the world. The contributors not only have hands-on experience in the field but often have developed the original approaches that they share with great attention to detail. The chapters provide a brief review of each field followed by a "cookbook? and handy illustrations. The collection of protocols includes the isolation and maintenance of stem cells from various species using
Anmerkung:
Description based upon print version of record.
,
Front Cover; Embryonic Stem Cells; Copyright Page; Dedication Page; Table of Contents; Contributors to Volume 418; Preface; Foreword; Volumes in Series; Section I: Derivation and Maintenance of Embryonic Stem Cells; Chapter 1: Murine Embryonic Stem Cells; Historical Overview; Factors Affecting the Efficiency of Mouse ES Cell Establishment; Factors Affecting the Contribution of Mouse ES Cell to Chimeric Embryos; Critical Events During Mouse ES Cell Establishment; Freezing of ES Cell Lines; Characterization; Protocols; Acknowledgments; References; Chapter 2: Bovine Embryonic Stem Cells
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IntroductionMaterials; Methods; References; Chapter 3: Avian Embryonic Stem Cells; Introduction; Selection of Culture Media and Routine Procedures; Derivation and Maintenance of Chicken Embryonic Stem Cell Lines; Genetic Modification of cES Cells; Production of Chimeric Chickens; Acquisition, Storage, and Quality Control of Eggs; Timeline of Chimera Production; Efficiency of Chimera Production Using Embryonic Stem Cells; Acknowledgments; References; Chapter 4: Zebrafish Embryonic Stem Cells; Introduction; Materials; Derivation of Zebrafish ES Cell Cultures
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Homologous Recombination in ES cells and Selection of Colonies Carrying a Targeted Plasmid InsertionAcknowledgments; References; Chapter 5: Human Embryonic Stem Cells; Introduction; Derivation of hES Cell Lines; Conclusion; Acknowledgments; References; Chapter 6: Embryonic Stem Cells from Morula; Introduction; Protocol for Isolation Human ES Cell Line from Morula; Characteristics of Morula-Derived hESC Lines; Protocol of Immunofluorescence Staining for TRA-2-39 or TRA-2-54 and Oct-4; Freezing Cell Lines; Protocol for Freezing Morula-Derived hESC Lines
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Procedure for Freezing Human Embryonic Stem CellsThawing hESC Lines; Acknowledgments; References; Chapter 7: Embryonic Stem Cells from Single Blastomeres; Introduction; Derivation of ES Cells from a Single Blastomere; Analysis of Cell Lines; References; Chapter 8: Embryonic Stem Cells from Parthenotes; Introduction; Mouse Parthenogenetic ESCs; Nonhuman Primate Parthenogenetic ESCs; The Importance of Choosing the Right Activation Protocol; Imprinting and Parthenogenesis; Protocols for Derivation of Parthenogenetic ESCs; Summary; Acknowledgments; References
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Chapter 9: Embryonic Stem Cells Using Nuclear TransferIntroduction; Somatic Cell Nuclear Transfer; Media Preparation; Culture Dishes and Incubator; Protocol; Derivation of ES Cells from Cloned Blastocysts; References; Section II: Differentiation of Embryonic Stem Cells; Chapter 10: Neural Stem Cells, Neurons, and Glia; Introduction; Protocols; Media and Reagents; Summary; Acknowledgments; References; Chapter 11: Retinal Pigment Epithelium; Introduction; Differentiation of hES Cells to RPE; Materials and Equipment; Harvesting RPE Cells; Culture and Properties of hES-Derived RPE
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RPE Culture Protocols
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English
Weitere Ausg.:
ISBN 9780123736482
Weitere Ausg.:
ISBN 012373648X
Sprache:
Englisch
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