UID:
almafu_9958128519502883
Umfang:
1 online resource (516 p.)
ISBN:
9786610751631
,
9781280751639
,
1280751630
,
9780080469645
,
0080469647
Serie:
Methods in enzymology, v. 420
Inhalt:
This is the third of three planned volumes in the Methods in Enzymology series on the topic of stem cells. This volume is a unique anthology of stem cell techniques written by experts from the top laboratories in the world. The contributors not only have hands-on experience in the field but often have developed the original approaches that they share with great attention to detail. The chapters provide a brief review of each field followed by a "cookbook? and handy illustrations. The collection of protocols includes the isolation and maintenance of stem cells from various species using
Anmerkung:
Description based upon print version of record.
,
Front Cover; Stem Cell Tools and Other Experimental Protocols; Copyright Page; Dedication; Table of Contents; Contributors to Volume 420; Preface; Foreword; Volumes in Series; Section I: In Vitro Experimentation and Research Tools; Chapter 1: Human Embryo Culture; Introduction; Human Embryo Development; Human Embryo Culture: General Considerations; Protocols; Results; Acknowledgments; References; Chapter 2: Characterization and Evaluation of Human Embryonic Stem Cells; Introduction; Characterization of Undifferentiated hESCs; Conclusion; Acknowledgments; References
,
Chapter 3: Feeder-Free Culture of Human Embryonic Stem CellsIntroduction; Methods for Feeder-Free Culture of hESCs; Acknowledgments; References; Chapter 4: Transgene Expression and RNA Interference in Embryonic Stem Cells; Retrovirus Expression Vectors and Embryonic Stem Cells; RNA Interference and ESCs; siRNA Expression Vector Design; Retrovirus Production; Retroviral and Lentiviral Gene Transfer into Mouse and Human ESCs; Transgene and siRNA Expression in Mouse and Human ESCs; Biotechnological and Medical Applications; Acknowledgments; References
,
Chapter 5: Lentiviral Vector-Mediated Gene Delivery into Human Embryonic Stem CellsIntroduction; Development of Lentiviral-Based Vectors; Design of HIV-1-Based Vectors; Transduction of hESCs by Lentiviral-Based Vectors; Potential Applications of Gene Delivery into hESCs by Lentiviral-Based Vectors; Design of HIV-1-Based Vectors for Transduction of hESCs; References; Chapter 6: The Use of Retroviral Vectors for Gene Transfer into Hematopoietic Stem Cells; Introduction; Viral Pseudotype; Vector Design; Packaging Cell Lines; Clinical Applications; Generation of Retrovirus Packaging Cell Lines
,
Summary and Future DirectionsAcknowledgments; References; Chapter 7: Engineering Embryonic Stem Cells with Recombinase Systems; Introduction; Site-Specific Recombination; Large Serine Recombinases; Designing Substrates for Site-Specific Recombination; Generation of Conditional Alleles; Recombinase-Mediated Cassette Exchange (RMCE); Molecular Switches; Protocols; References; Chapter 8: Gene Trapping in Embryonic Stem Cells; Introduction; Common Types of Gene Trap Vectors in IGTC; A User's Guide to the International Gene Trap Consortium Resource; Investigator-Initiated Screens; References
,
Chapter 9: GeneChips in Stem Cell ResearchIntroduction; Protocol; ESC Differentiation; ESC Genes and In Vivo Regeneration; Cell Screening; Integration and Meta-Analysis of GeneChip and Microarray Data; Applications to Cancer; Conclusion; Summary; Acknowledgments; References; Further Reading; Chapter 10: Microarray Analysis of Stem Cells and Differentiation; Introduction; Overview of Microarray Technology; Total RNA Isolation and Clean-Up; RNA Amplification; Direct Labeling of RNA for Microarray Hybridization; Indirect Labeling of RNA for Microarray Hybridization; Microarray Hybridization
,
Array-Based Comparative Genomic Hybridization (Array CGH)
,
English
Weitere Ausg.:
ISBN 9780123736512
Weitere Ausg.:
ISBN 012373651X
Sprache:
Englisch
Bookmarklink
